Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Occupational Exposure to Pesticides in Tobacco Fields: The Integrated Evaluation of Nutritional Intake and Susceptibility o Genomic and Epigenetic Instability

Texto completo
Autor(es):
Mostrar menos -
Silva Kahl, Vivian F. [1] ; Dhillon, Varinderpal [2] ; Fenech, Michael [2] ; de Souza, Melissa Rosa [1] ; da Silva, Fabiane Nitzke [1] ; Possa Marroni, Norma Anair [3, 4] ; Nunes, Emilene Arusievicz [5] ; Cerchiaro, Giselle [5] ; Pedron, Tatiana [6] ; Batista, Bruno Lemos [6] ; Cappetta, Monica [7] ; Martinez-Lopez, Wilner [8] ; Simon, Daniel [9] ; da Silva, Juliana [1]
Número total de Autores: 14
Afiliação do(s) autor(es):
[1] Lutheran Univ Brazil ULBRA, Postgrad Program Cellular & Mol Biol Appl Hlth PP, Lab Toxicol Genet, Av Farroupilha 8001, Canoas, RS - Brazil
[2] CSIRO, Hlth & Biosecur Flagship, Gate 13 Kintore Ave, Adelaide, SA - Australia
[3] Lutheran Univ Brazil ULBRA, Postgrad Program Cellular & Mol Biol Appl Hlth PP, Lab Oxidat Stress, Av Farroupilha 8001, Canoas, RS - Brazil
[4] Fed Univ Rio Grande do Sul UFRGS, Lab Expt Hepatol Physiol, Rua Ramiro Barcelos 2350, Porto Alegre, RS - Brazil
[5] Fdn Fed Univ ABC UFABC, Postgrad Program Biosyst, Av Estados 5001, Santo Andre, SP - Brazil
[6] Fdn Fed Univ ABC UFABC, Postgrad Program Sci & Technol Chem, Av Estados 5001, Santo Andre, SP - Brazil
[7] Univ Republica, Med Sch, Genet Dept, Lab Genet Epidemiol, Gral Flores 2125, Montevideo - Uruguay
[8] Inst Invest Biol Clemente Estable, Epigenet & Genom Instabil Lab, Montevideo - Uruguay
[9] Lutheran Univ Brazil ULBRA, Postgrad Program Cellular & Mol Biol Appl Hlth PP, Lab Human Mol Genet, Av Farroupilha 8001, Canoas, RS - Brazil
Número total de Afiliações: 9
Tipo de documento: Artigo Científico
Fonte: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; 2018.
Citações Web of Science: 2
Resumo

Pesticides used at tobacco fields are associated with genomic instability, which is proposed to be sensitive to nutritional intake and may also induce epigenetic changes. We evaluated the effect of dietary intake and genetic susceptibility polymorphisms in MTHFR (rs1801133) and TERT (rs2736100) genes on genomic and epigenetic instability in tobacco farmers. Farmers, when compared to a nonexposed group, showed increased levels of different parameters of DNA damage (micronuclei, nucleoplasmic bridges, and nuclear buds), evaluated by cytokinesis-block micronucleus cytome assay. Telomere length (TL) measured by quantitative PCR was shorter in exposed individuals. Global DNA methylation was significantly decreased in tobacco farmers. The exposed group had lower dietary intake of fiber, but an increase in cholesterol; vitamins such as B-6, B-12 , and C; beta-carotene; and alpha-retinol. Several trace and ultratrace elements were found higher in farmers than in nonfarmers. The MTHFR CT/TT genotype influenced nucleoplasmic bridges, nuclear buds, and `11 in the exposed group, whereas TERT GT/TT only affected micronucleus frequency. We observed a positive correlation of TL and lipids and an inverse correlation of TL and fibers. The present data suggest an important role of dietary intake and subjects' genetic susceptibility to xenobiotics-induced damages and epigenetic alterations in tobacco farmers occupationally exposed to mixtures of pesticides. (AU)

Processo FAPESP: 14/05151-0 - Arsênio e arroz: monitoramento e estudos de (bio)remediação para segurança alimentar
Beneficiário:Bruno Lemos Batista
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores