In vivo investigation of the volatile metabolome of antiphytopathogenic yeast strains active against Penicillium digitatum using comprehensive two-dimensional gas chromatography and multivariate data analysis

Autor(es)

Belinato de Souza, Joao Raul ^{[1, 2]} ; Kupper, Katia Cristina ^[3] ; Augusto, Fabio ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, UNICAMP, CP 6154, BR-13083970 Campinas, SP - Brazil

^[2] Natl Inst Bioanalyt Sci & Technol INCTBio, CP 6154, BR-13083970 Campinas, SP - Brazil

^[3] Campinas Agron Inst, Sylvio Moreira Citrus Res Ctr, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 141, p. 362-368, SEP 2018.
• Citações Web of Science: 2

Resumo

Penicillium digitatum is the responsible for green mold decay on citrus fruits, which contributes to economic losses in agriculture industry during storage, transportation and in the market. Biocontrol agents may be a viable and low-cost alternative in the control of different plant pathogens: metabolites from some yeast strains can prevent infection, decreasing host tissue colonization and reducing pathogen survival and sporulation with varying degrees of efficiency. Eight S. cerevisae strains and one Candida stellimalicola strain all were evaluated in vitro as biological control agents against P. digitatum. After in vitro tests the headspace of yeast strains were extracted in vivo using an optimized HS-SPME + GC x GC MS method. The correlation between antiphytopathogenic activity with the volatile metabolite profiles was assessed using principal component analysis. Nine compounds were associated with the volatile metabolome of strains with higher antifungal action: ethyl propionate, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl butyrate, 3-methylbutyl acetate, 3-methylbutyl propionate, n-pentyl butyrate, phenylethyl alcohol and 2-phenethyl acetate. This study allowed for the first time a comprehensive overview of the effect of individual volatile metabolites from yeast strains on P. digitatum infection. (AU)

• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Lauro Tatsuo Kubota
• Linha de fomento: Auxílio à Pesquisa - Temático