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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The Aspergillus nidulans Pyruvate Dehydrogenase Kinases Are Essential To Integrate Carbon Source Metabolism

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Autor(es):
Annick Ries, Laure Nicolas [1] ; de Assis, Leandro Jose [1] ; Santos Rodrigues, Fernando Jose [2] ; Caldana, Camila [3] ; Rocha, Marina Campos [4] ; Malavazi, Iran [4] ; Bayram, Ozgur [5] ; Goldman, Gustavo H. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, FCFRP, Bloco Q, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Minho, Inst Invest Ciencias Vida & Saude, Campus Gualtar, P-4710057 Braga - Portugal
[3] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Sao Paulo - Brazil
[4] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, Sao Paulo - Brazil
[5] Maynooth Univ, Dept Biol, Maynooth, Kildare - Ireland
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: G3-GENES, GENOMES, GENETICS; v. 8, n. 7, p. 2445-2463, JUL 2018.
Citações Web of Science: 2
Resumo

The pyruvate dehydrogenase complex (PDH), that converts pyruvate to acetyl-coA, is regulated by pyruvate dehydrogenase kinases (PDHK) and phosphatases (PDHP) that have been shown to be important for morphology, pathogenicity and carbon source utilization in different fungal species. The aim of this study was to investigate the role played by the three PDHKs PkpA, PkpB and PkpC in carbon source utilization in the reference filamentous fungus Aspergillus nidulans, in order to unravel regulatory mechanisms which could prove useful for fungal biotechnological and biomedical applications. PkpA and PkpB were shown to be mitochondrial whereas PkpC localized to the mitochondria in a carbon source-dependent manner. Only PkpA was shown to regulate PDH activity. In the presence of glucose, deletion of pkpA and pkpC resulted in reduced glucose utilization, which affected carbon catabolite repression (CCR) and hydrolytic enzyme secretion, due to de-regulated glycolysis and TCA cycle enzyme activities. Furthermore, PkpC was shown to be required for the correct metabolic utilization of cellulose and acetate. PkpC negatively regulated the activity of the glyoxylate cycle enzyme isocitrate lyase (ICL), required for acetate metabolism. In summary, this study identified PDHKs important for the regulation of central carbon metabolism in the presence of different carbon sources, with effects on the secretion of biotechnologically important enzymes and carbon source-related growth. This work demonstrates how central carbon metabolism can affect a variety of fungal traits and lays a basis for further investigation into these characteristics with potential interest for different applications. (AU)

Processo FAPESP: 14/00789-6 - Caracterização funcional de fosfatases de Aspergillus nidulans envolvidas no metabolismo da glicose
Beneficiário:Leandro Jose de Assis
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/07870-9 - A influência de proteínas quinases ativadas por mitógenos (MAPK) na expressão de determinantes genéticos importantes para a virulência de Aspergillus fumigatus
Beneficiário:Gustavo Henrique Goldman
Linha de fomento: Auxílio à Pesquisa - Temático