LLC tumor cells-derivated factors reduces adipogenesis in co-culture system

Autor(es)

Lopes, Magno Alves ^[1] ; Franco, Felipe Oliveira ^[1] ; Henriques, Felipe ^[2] ; Peres, Sidney Barnabe ^[3] ; Batista, Jr., Miguel Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Mogi das Cruzes, Ctr Integrated Biotechnol, Lab Adipose Tissue Biol, Sao Paulo - Brazil

^[2] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA - USA

^[3] Univ Estadual Maringa, Dept Physiol Sci, Maringa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HELIYON; v. 4, n. 7 JUL 2018.
• Citações Web of Science: 1

Resumo

Cancer cachexia (CC) is a multifactorial syndrome with an unknown etiology. The primary symptom is the progressive reduction of the body weight. Recently, down-regulation of adipogenic and lipogenic genes were demonstrated to be early affected during cachexia progression in adipose tissue (AT), resulting in AT remodeling. Thus, this study aimed to evaluate in a co-culture system the influence of the Lewis Lung Carcinoma (LLC) tumor cells (c/c-LLC) in an established preadipocyte cell line 3T3-L1 adipogenic capacity. c/c-LLC in the presence of 3T3-L1 caused a reduction in lipids accumulation, suggesting that secretory tumor cells products may affect adipogenesis. Interestingly, a very early (day 2) downregulation of proliferator-activated receptor gamma (PPAR gamma) and CCAAT/enhancer-binding protein alpha (C/EBP alpha), followed by late genes (day 4 and 8), adiponectin, perilipin, and fatty acid-binding protein 4 (FABP4). Caspase-3 expression was increased on the last day of cell differentiation; it occurred in the expression of pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha). Overall, our results suggest that LLC secretory products impair adipocyte differentiation in a co-culture system and increased apoptosis. In summary, our study has shown the inhibition of the adipogenic process in the 3T3-L1 co-culture system with LLC cells. (AU)

• Processo FAPESP: 10/51078-1 - Bases moleculares da caquexia: adipogênese e remodelagem da matriz extracelular do tecido adiposo branco de pacientes com câncer gastrointestinal
• Beneficiário: Miguel Luiz Batista Junior
• Linha de fomento: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/19259-0 - Repercussões do remodelamento do tecido adiposo durante a síndrome de caquexia em pacientes com câncer gastrointestinal: potencial envolvimento do receptor TLR4 na modulação do browning no TAB
• Beneficiário: Miguel Luiz Batista Junior
• Linha de fomento: Auxílio à Pesquisa - Regular