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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS

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Autor(es):
Saleh, Mayra A. D. [1] ; Dos Santos, Luan S. [1] ; Berto, Dirlei A. [1] ; Amorim, Alessandro B. [2] ; Costa, Vladimir E. [3] ; Ducatti, Carlos [3]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Dept Prod Anim, Fac Med Vet & Zootecnia, Rua Dr Jose Barbosa Barros 1780, BR-18610307 Botucatu, SP - Brazil
[2] Univ Fed Mato Grosso, Inst Ciencias Agr & Tecnol, Ave Estudantes 5005, BR-78735902 Rondonopolis, MT - Brazil
[3] Univ Estadual Paulista, Inst Biociencias, Ctr Isotopos Estaveis Ambientais, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Anais da Academia Brasileira de Ciências; v. 90, n. 2, 1, p. 2469-2478, AUG 2018.
Citações Web of Science: 0
Resumo

Isotope-ratio mass spectrometry (IRMS) is a potential tool that provides time-integrated estimate of assimilated and not just ingested nutrients. This feature turned possible its application to evaluate the effects of dietary nucleotides and glutamate on carbon turnover (delta C-13) in the brain of weanling piglets. Eighty-seven piglets weaned at 21-day-old were used, being three piglets slaughtered at day-0 (prior to diet-switch) and, the remaining 84 randomly assigned in a complete block design with a 2 x 2 factorial arrangement of treatments (two nucleotide levels: 0% and 0.1% and, two glutamate levels: 0% and 1%). On trial days 3, 6, 9, 14, 21, 35 and 49, three piglets per treatment were also slaughtered. The samples were analyzed by IRMS and adjusted to the first-order equation by a nonlinear regression analysis using NLIN procedure of SAS, in order to obtain the exponential graphics. The carbon turnover (t(95%)) verified for cerebral tissue was faster (P<0.05) for diet containing glutamate in comparison to other diets, supporting the fact that glutamate contributed to develop the piglets' brain, due to the fastest incorporation of C-13-atoms in this tissue at post-weaning phase, despite the energy deficit experienced by them. (AU)

Processo FAPESP: 13/25616-4 - Capacidade de adaptação de leitões ao desmame recebendo dietas suplementadas com nucleotídeos e ácido glutâmico
Beneficiário:Dirlei Antonio Berto
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/13169-6 - Troca isotópica do carbono (´13C) em órgãos e músculos de leitões recebendo dietas com nucleotídeos e glutamato
Beneficiário:Mayra Anton Dib Saleh
Linha de fomento: Bolsas no Brasil - Pós-Doutorado