Detrimental effects detected in exfoliated buccal cells from anesthesiology medical residents occupationally exposed to inhalation anesthetics: An observational study

Autor(es)

Braz, Mariana G. ^[1] ; Souza, Katina M. ^[1] ; Lucio, Lorena M. C. ^[1] ; Di Renzo, Giulia C. C. ^[1] ; Feliciano, Luciana M. ^[2] ; Marcondes, Joao Paulo C. ^[2] ; Oliver Chen, C. -Y. ^[3] ; Braz, Jose Reinaldo C. ^[1] ; Braz, Leandro G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Anesthesiol, Fac Med, Botucatu, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Genet, Botucatu, SP - Brazil

^[3] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 - USA

• Tipo de documento: Artigo Científico
• Fonte: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 832, p. 61-64, AUG 2018.
• Citações Web of Science: 1

Resumo

Operating room professionals are scarcely aware of their individual occupational exposure to waste anesthetic gases (WAGs). Medical residents spend several hours per day in operating rooms and consequently experience occupational exposure to WAGs. Considering that no studies have yet evaluated the potential toxicity in medical residents exposed to WAGs using the buccal micronucleus cytome (BMCyt) assay, this pioneering study aimed to compare the BMCyt assay markers, including DNA damage, cell proliferation, and cell death in the exfoliated buccal cells of surgery and anesthesiology residents occupationally exposed to WAGs. The study enrolled a total of 60 physicians, including internal medicine residents (unexposed group), and residents from surgery and anesthesiology programs who were occupationally exposed to sevoflurane, isoflurane and nitrous oxide. WAGs were measured, and the mean values were higher than the international recommendation. The anesthesiology residents (high exposure) showed statistically significant lower frequencies of basal cells, and statistically significant higher frequencies of micronuclei, karyorrhexis, pyknosis, and differentiated cells than did the unexposed group; karyolysis frequencies were significantly higher in anesthesiology residents than were those in the unexposed group or in surgical residents (low exposure). The findings suggest a genetic risk for young professionals exposed to WAGs at the beginning of their careers. Thus, exposure to high WAGs concentrations leads to impairment of the buccal cell proliferative potential, genomic instability and cell death, especially in anesthesiology residents, demonstrating an early impact on their health. (AU)

• Processo FAPESP: 13/21130-0 - Correlação entre concentração de resíduos de gases anestésicos com estresse oxidativo e resposta imunológica em residentes médicos ocupacionalmente expostos
• Beneficiário: Mariana Gobbo Braz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/05084-8 - Avaliação de danos no material genético em anestesiologistas
• Beneficiário: Leandro Gobbo Braz
• Modalidade de apoio: Auxílio à Pesquisa - Regular