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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Inactivation of phoPQ genes attenuates Salmonella Gallinarum biovar Gallinarum to susceptible chickens

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Autor(es):
Rodrigues Alves, Lucas Bocchini [1] ; de Freitas Neto, Oliveiro Caetano [2] ; Alves Batista, Diego Felipe [1] ; Barbos, Fernanda de Oliveira [1] ; Rubio, Marcela da Silva [1] ; Secundo de Souza, Andrei Itajahy [1] ; de Almeida, Adriana Maria [1] ; Barrow, Paul Andrew [3] ; Berchieri Junior, Angelo [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ Unesp, Sch Agr & Vet Sci, Post Grad Program Agr & Livestock Microbiol, Jaboticabal, SP - Brazil
[2] Univ Fed Paraiba, Dept Vet Sci, POB 58397-000, Areia, MG - Brazil
[3] Univ Nottingham, Sch Vet Med & Sci, Loughborough, Leics - England
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Microbiology; v. 49, n. 3, p. 601-606, JUL-SEP 2018.
Citações Web of Science: 0
Resumo

Salmonella Gallinarum is a host-restrict pathogen that causes fowl typhoid, a severe systemic disease that is one of the major concerns to the poultry industry worldwide. When infecting the bird, SG makes use of evasion mechanisms to survive and to replicate within macrophages. In this context, phoPQ genes encode a two-component regulatory system (PhoPQ) that regulates virulence genes responsible for adaptation of Salmonella spp. to antimicrobial factors such as low pH, antimicrobial peptides and deprivation of bivalent cations. The role of the mentioned genes to SG remains to be investigated. In the present study a phoPQ-depleted SG strain (SG Delta phoPQ) was constructed and its virulence assessed in twenty-day-old laying hens susceptible to fowl typhoid. SG Delta phoPQ did cause neither clinical signs nor mortality in birds orally challenged, being non-pathogenic. Furthermore, this strain was not recovered from livers or spleens. On the other hand, chickens challenged subcutaneously with the mutant strain had discreet to moderate pathological changes and also low bacterial counts in liver and spleen tissues. These findings show that SG Delta phoPQ is attenuated to susceptible chickens and suggest that these genes are important during chicken infection by SG. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. (AU)

Processo FAPESP: 16/10369-0 - Patogenicidade de Salmonella spp. contendo deleções nos genes PhoPQ, fliD e CLPP em aves comerciais
Beneficiário:Angelo Berchieri Junior
Linha de fomento: Auxílio à Pesquisa - Regular