Compositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakes

Autor(es)

Galizio, Nathalia da Costa ^[1] ; Serino-Silva, Caroline ^{[1, 2]} ; Stuginski, Daniel Rodrigues ^[1] ; Estima Abreu, Patricia Antonia ^[3] ; Sant'Anna, Savio Stefanini ^[1] ; Grego, Kathleen Fernandes ^[1] ; Tashima, Alexandre Keiji ^[4] ; Tanaka-Azevedo, Anita Mitico ^{[1, 2]} ; de Morais-Zani, Karen ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Herpetol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Butantan, Inst Pesquisas Tecnol, Interunidades Biotecnol, Sao Paulo - Brazil

^[3] Inst Butantan, Lab Bacteriol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOMICS; v. 186, p. 56-70, AUG 30 2018.
• Citações Web of Science: 3

Resumo

Intraspecific venom variability has been extensively reported in a number of species and is documented to be the result of several factors. However, current evidence for snake venom variability related to captivity maintenance is controversial. Here we report a compositional and functional investigation of individual and pooled venoms from long-term captive (LTC) and recently wild-caught (RWC) B. jararaca snakes. The composition of individual venoms showed a remarkable variability in terms of relative abundance of toxins (evidenced by 1-DE and RPHPLC), enzymatic activities (proteolytic, PLA(2), and LAAO) and coagulant activity, even among captive specimens. Thus, no compositional and functional pattern could be established to assign each individual venom to a specific group. Conversely, pooled venom from LTC and RWC snakes showed no significant differences regarding protein composition (characterized by 1-DE and shotgun proteomics), enzymatic activities (proteolytic, PLA2 and LAAO) and biological function (coagulant, hemorrhagic and lethal activities), except for edematogenic activity, which was more prominent in RWC venom pool. Additionally, both pooled venoms displayed similar immunoreactivity with the bothropic antivenom produced by Instituto Butantan. Taken together, our results highlight the complexity and the high intraspecific variation of B. jararaca venom, that is not influenced at a discernible extent by captivity maintenance. (AU)

• Processo FAPESP: 14/11108-0 - Estudo comparativo da composição das biomoléculas dos venenos das serpentes Bothrops jararaca e Crotalus durissus terrificus nascidas e mantidas no Instituto Butantan e recém-chegadas da natureza
• Beneficiário: Karen de Morais Zani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/09976-9 - Comparação do veneno de serpentes Bothrops jararaca em cativeiro e recém-chegadas da natureza
• Beneficiário: Nathália da Costa Galizio
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/01890-0 - Estudo comparativo da composição do veneno das serpentes Crotalus durissus terrificus nascidas em cativeiro no Laboratório de Herpetologia do Instituto Butantan e do veneno crotálico de referência nacional
• Beneficiário: Anita Mitico Tanaka-Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/16908-2 - Caracterização proteômica e das atividades enzimáticas e fisiopatológicas do veneno das espécies de serpentes que compõem o grupo Bothrops neuwiedi
• Beneficiário: Karen de Morais Zani
• Modalidade de apoio: Auxílio à Pesquisa - Regular