Pathogenesis of the Candida parapsilosis Complex in the Model Host Caenorhabditis elegans

Autor(es)

Remondi Souza, Ana Carolina ^{[1, 2]} ; Fuchs, Beth Burgwyn ^[1] ; Alves, Viviane de Souza ^[3] ; Jayamani, Elamparithi ^[1] ; Colombo, Arnaldo Lopes ^[2] ; Mylonakis, Eleftherios ^[1]

Afiliação do(s) autor(es)

^[1] Brown Univ, Rhode Isl Hosp, Alpert Med Sch, Div Infect Dis, Providence, RI 02903 - USA

^[2] Fed Univ Sao Paulo UNIFESP, Div Infect Dis, Special Mycol Lab, BR-04039032 Sao Paulo, SP - Brazil

^[3] Univ Fed Minas Gerais, Biol Sci Inst, Microbiol Dept, Microorganisms Cell Biol Lab, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENES; v. 9, n. 8 AUG 2018.
• Citações Web of Science: 4

Resumo

Caenorhabditis elegans is a valuable tool as an infection model toward the study of Candida species. In this work, we endeavored to develop a C. elegans-Candida parapsilosis infection model by using the fungi as a food source. Three species of the C. parapsilosis complex (C. parapsilosis (sensu stricto), Candida orthopsilosis and Candida metapsilosis) caused infection resulting in C. elegans killing. All three strains that comprised the complex significantly diminished the nematode lifespan, indicating the virulence of the pathogens against the host. The infection process included invasion of the intestine and vulva which resulted in organ protrusion and hyphae formation. Importantly, hyphae formation at the vulva opening was not previously reported in C. elegans-Candida infections. Fungal infected worms in the liquid assay were susceptible to fluconazole and caspofungin and could be found to mount an immune response mediated through increased expression of cnc-4, cnc-7, and fipr-22/23. Overall, the C. elegans-C. parapsilosis infection model can be used to model C. parapsilosis host-pathogen interactions. (AU)

• Processo FAPESP: 17/02203-7 - Rede multidisciplinar internacional para caracterização de aspectos microbiológicos e da história natural de Infecções Fúngicas Invasivas (IFI) por espécies do gênero Candida
• Beneficiário: Arnaldo Lopes Colombo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/04769-4 - Complexo Candida parapsilosis: peculiaridades epidemiológicas, virulência e mecanismos de resistência aos antifúngicos.
• Beneficiário: Ana Carolina Remondi Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07405-6 - Análise da patogenicidade das espécies do complexo C. parapsilosis utilizando modelos invertebrados de infecção
• Beneficiário: Ana Carolina Remondi Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado