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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genotoxic effects of BnSP-6, a Lys-49 phospholipase A(2) (PLA(2)) homologue from Bothrops pauloensis snake venom, on MDA-MB-231 breast cancer cells

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Autor(es):
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Silva, Makswell Almeida [1] ; Lopes, Daiana Silva [1] ; Teixeira, Samuel Cota [2] ; Cirilo Gimenes, Sarah Natalie [1] ; Vasconcelos Azevedo, Fernanda Van Petten [1] ; Polloni, Lorena [3] ; Borges, Bruna Cristina [4] ; da Silva, Marcelo Santos [5] ; Barbosa, Marcelo Jose [4] ; de Oliveira Junior, Robson Jose [3] ; Elias, Maria Carolina [5] ; da Silva, Claudio Vieira [2] ; Geraldo Yoneyama, Kelly Aparecida [1] ; Rodrigues, Veridiana de Melo [1] ; Rodrigues, Renata Santos [1]
Número total de Autores: 15
Afiliação do(s) autor(es):
[1] Univ Fed Uberlandia, UFU, Inst Biotechnol, Lab Biochem & Anim Toxins, Uberlandia, MG - Brazil
[2] Univ Fed Uberlandia, Biomed Sci Inst, Dept Immunol, Lab Trypanosomatids, UFU, Uberlandia, MG - Brazil
[3] Univ Fed Uberlandia, Genet & Biochem Inst, Lab Cytogenet Anim, UFU, Uberlandia, MG - Brazil
[4] Univ Fed Uberlandia, Biomed Sci Inst, Lab Tumor Biomarkers & Osteoimmunol, UFU, Uberlandia, MG - Brazil
[5] Butantan Inst, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Macromolecules; v. 118, n. A, p. 311-319, OCT 15 2018.
Citações Web of Science: 2
Resumo

Herein we evaluated the genotoxic effects of BnSP-6, a Lys-49 phospholipase A(2) (PLA(2)) from Bothrops pauloensis, on breast cancer cells. BnSP-6 was able to induce a higher cytotoxic and genotoxic activity in MDA-MB-231 cells, when compared to MCF10A (a non-tumorigenic breast cell line), suggesting that this protein presented a possible preference for cancer cells. BnSP-6 inhibited MDA-MB-231 proliferation at 24, 48 and 72 h. In addition, BnSP-6 induced significant increase in the percentage of TUNEL-positive cells, a marker of DNA damage. To obtain novel insight into the direct DNA damage interference in MDA-MB-231 survival and proliferation, we evaluated cell cycle progression. BnSP-6 produced a significant decrease in 2N (GI) and an increase in the G2/M phase and this capacity is likely related to the modulation of expression of progression cell cycle-associated genes (CCND1, CCNE1, CDC25A, CHEK2, E2F1, CDH-1 and NF-kB). Taken together, these results indicate that BnSP-6 induces DNA damage in breast cancer cells and is an attractive model for developing innovative therapeutic agents against breast cancer. (C) 2018 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
Beneficiário:Hugo Aguirre Armelin
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 14/24170-5 - Dinâmica da replicação do DNA em Trypanosoma cruzi: caracterização do licenciamento e da taxa de replicação
Beneficiário:Marcelo Santos da Silva
Linha de fomento: Bolsas no Brasil - Pós-Doutorado