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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Rare RELN variants affect Reelin-DAB1 signal transduction in autism spectrum disorder

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Autor(es):
Sanchez-Sanchez, Sandra M. [1, 2] ; Magdalon, Juliana [2] ; Griesi-Oliveira, Karina [2] ; Yamamoto, Guilherme L. [1] ; Santacruz-Perez, Carolina [3] ; Fogo, Mariana [1, 2] ; Passos-Bueno, Maria Rita [1] ; Sertie, Andrea L. [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Sao Paulo - Brazil
[2] Hosp Israelita Albert Einstein, Ctr Expt Res, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Human mutation; v. 39, n. 10, p. 1372-1383, OCT 2018.
Citações Web of Science: 2
Resumo

The Reelin-DAB1 signaling pathway plays a crucial role in regulating neuronal migration and synapse function. Although many rare heterozygous variants in the Reelin gene (RELN) have been identified in patients with autism spectrum disorder (ASD), most variants are still of unknown clinical significance. Also, genetic data suggest that heterozygous variants in RELN alone appear to be insufficient to cause ASD. Here, we describe the identification and functional characterization of rare compound heterozygous missense variants in RELN in a patient with ASD in whom we have previously reported hyperfunctional mTORC1 signaling of yet unknown etiology. Using iPSC-derived neural progenitor cells (NPCs) from this patient, we provide experimental evidence that the identified variants are deleterious and lead to diminished Reelin secretion and impaired Reelin-DAB1 signal transduction. Also, our results suggest that mTORC1 pathway overactivation may function as a second hit event contributing to downregulation of the Reelin-DAB1 cascade in patient-derived NPCs, and that inhibition of mTORC1 by rapamycin attenuates Reelin-DAB1 signaling impairment. Taken together, our findings point to an abnormal interplay between Reelin-DAB1 and mTORC1 networks in nonsyndromic ASD. (AU)

Processo FAPESP: 14/25646-3 - Investigação dos efeitos moleculares e celulares de mutações no gene Reln identificadas em pacientes com Transtornos do Espectro Autista
Beneficiário:Andréa Laurato Sertié
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/50138-4 - Investigação do papel do sistema do complemento no desenvolvimento cerebral e nos Transtornos do Espectro Autista
Beneficiário:Andréa Laurato Sertié
Linha de fomento: Auxílio à Pesquisa - Regular