Stress-related salivary proteins affect the production of volatile sulfur compounds by oral bacteria

Autor(es)

de Lima, Patricia Oliveira ^[1] ; Nani, Bruno Dias ^[1] ; Almeida, Barbara ^[1] ; Marcondes, Fernanda Klein ^[1] ; Groppo, Francisco Carlos ^[1] ; Alves de Moraes, Antonio Bento ^[1] ; Franz-Montan, Michelle ^[1] ; Cogo-Mueller, Karina ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Dept Physiol Sci, Piracicaba Dent Sch, Piracicaba, SP - Brazil

^[2] Univ Campinas Unicamp, Fac Pharmaceut Sci, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 24, n. 7, p. 1358-1366, OCT 2018.
• Citações Web of Science: 1

Resumo

ObjectiveTo determine whether stress-related substances and sex hormones influence the growth and in vitro production of volatile sulfur compounds (VSCs) by Solobacterium moorei and Fusobacterium nucleatum. Materials and MethodsBacteria growth and VSCs production were evaluated in the presence of alpha-amylase, beta-defensin-2, mucin, estradiol, and progesterone. Growth was evaluated by colony counting, and the production of the VSCs hydrogen sulfide (H2S) and methyl mercaptan (CH3SH) was measured using the Oral Chroma instrument. ResultsMucin induced the production of H2S by both bacteria, but had a slight inhibitory effect on CH3SH production by F.nucleatum. It also increased the viability of F.nucleatum. Alpha-amylase increased H2S production by S.moorei and CH3SH production by F.nucleatum, but had no effect on H2S production by F.nucleatum. No substance altered the viability of S.moorei. No effects of beta-defensin-2, estradiol, or progesterone were observed. ConclusionThe salivary stress-related proteins mucin and alpha-amylase altered VSCs production by F.nucleatum and S.moorei, favoring H2S production. These findings are a step toward understanding the relation between stress and increased amounts of H2S. (AU)

• Processo FAPESP: 13/26691-0 - Relação entre estresse e microbiota oral: efeito sobre a produção de compostos sulfurados voláteis e papel da beta-defensina
• Beneficiário: Bruno Dias Nani
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/50419-2 - Comportamento e saúde bucal: perspectivas interdisciplinares e aspectos emocionais no processo saúde-doença
• Beneficiário: Antonio Bento Alves de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/11481-2 - Relação entre estresse, hormônios sexuais femininos e microbiota bucal: efeito sobre produção de compostos sulfurados voláteis e papel da beta-defensina
• Beneficiário: Patricia Oliveira de Lima
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado