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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Activation of TP receptors induces high release of PGI(2) in coronary arteries of renal hypertensive rats

Texto completo
Autor(es):
Paula, T. D. [1] ; Silva, B. R. [1] ; Grando, M. D. [1] ; Souza, H. C. D. [2] ; Bendhack, L. M. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Physiol, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY; v. 122, p. 125-133, SEP 2018.
Citações Web of Science: 1
Resumo

Aim: To investigate the molecular mechanisms and cellular signaling pathways involved in the activation of TP receptors and the consequent induction of contractile responses in coronary arteries of renal hypertensive (2K-1C) rats. Methods and results: The coronary perfusion pressure (CPP) was lower in 2K-1C rats during increased coronary flow as measured by the Langendorff technique. The coronary contraction and relaxation were evaluated by vascular reactivity studies, and the molecular mechanisms were investigated on the basis of the protein expression of TP receptors, Cav-1, eNOS, COX-1, and COX-2, as measured by Western blot. The levels of eicosanoids were determined by ELISA immunoassay and analyzed by reverse-phase HPLC coupled to electrospray ionization mass spectrometry (HPLC-MS/MS). The metabolites from NO production were evaluated by the Griess reaction. The coronary arteries of 2K-1C rats expressed COX-2 to a larger extent and TP receptors to a lesser extent than the coronary arteries of normotensive (2K) rats. Selective COX-1 and non-selective COX inhibitors reversed the reduction in the contraction induced by TP receptors in the coronary arteries of 2K-1C rats. U46619, an agonist of TP receptors, induced a contractile response that was relaxed by acetylcholine (ACh). In the coronary arteries of 2K-1C rats, this ACh-induced relaxation depended on COX. The activation of TP receptors increased the production of PGI(2) in the coronary arteries of 2K-1C rats. The results demonstrated that increased COX signaling in the coronary arteries of 2K-1C rats mediated the low levels of CPP, the contraction induced by the activation of TP receptors, and the endothelium-dependent relaxation. The vasodilator PGI(2) seemed to be the major product. Conclusion: Activation of TP receptors increases production of PGI(2) in coronary arteries of 2K-1C rats. (AU)

Processo FAPESP: 15/17080-2 - Efeitos do estiramento sobre a vasoconstrição modulada pelo endotélio em aorta e coronária de ratos hipertensos renais
Beneficiário:Lusiane Maria Bendhack
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/00658-1 - Novos aspectos funcionais dos eicosanóides
Beneficiário:Lúcia Helena Faccioli
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários