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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Magneto low-density nanoemulsion (MLDE): A potential vehicle for combined hyperthermia and photodynamic therapy to treat cancer selectively

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Autor(es):
Pellosi, Diogo Silva [1, 2] ; Macaroff, Patricia Pereira [2] ; Morais, Paulo Cesar [3] ; Tedesco, Antonio Claudio [2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Selo Paulo UNIFESP, Lab Hibrid Mat, Dept Ciencias Exatas & Terra, Rua Sao Nicolau 210, BR-09913030 Diadema - Brazil
[2] Univ Sao Paulo, Ctr Nanotechnol & Tissue Engn, Photobiol & Photomed Res Grp, Dept Chem, Fac Philosophy Sci & Letters Ribeirao P, Sao Paulo - Brazil
[3] Univ Brasilia, Lab Opt Spect, Inst Phys, BR-70919970 Brasilia, DF - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 92, p. 103-111, NOV 1 2018.
Citações Web of Science: 4
Resumo

In this paper, we introduce a new drug delivery system (DDS) called magneto low-density nanoemulsion (MLDE), which can carry maghemite nanoparticles and Chlorin e6 as an active photosensitizer drug. This design can enhance tumor damage after minor heat dissipation and/or minimum visible light photosensitization doses by classical magnetic hyperthermia (MHT) and photodynamic therapy (PDT), respectively. We establish protocols to prepare the MLDE and to load the drug combination onto it. The MLDE prepared herein is nanometric ( < 200 nm), has high encapsulation efficiency, and is stable for at least 12 months in water dispersions. Flow cytometry results demonstrated that MLDE presents targeted selectivity toward the MCF-7 breast cancer cell line but not in NHI-3T3 mouse fibroblast cell lines, because the MCF-7 cancer cell surface contains overexpressed low density lipoprotein (LDL) receptors. Despite this targeted effect, MHT or PDT alone does not prompt significant antiproliferative outcomes. On the other hand, MHT and PDT in combination induce a strong and synergic action on MCF-7 cells and reduce the cell viability. In conclusion, the developed MLDE deserves further investigation because it is biocompatible, displays good encapsulation efficiency, and is highly stable. Moreover, it is selectively taken up by cancer cell surfaces with receptor recognition based on LDL receptor overexpression, which potentiates the action of combined MHT and PDT. (AU)

Processo FAPESP: 16/00389-3 - Nanopartículas multifuncionais para a entrega sítio-específica da temozolomida e verteporfina para a terapia combinada de tumores cerebrais
Beneficiário:Diogo Silva Pellosi
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 15/18684-9 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
Beneficiário:Leonardo Barcelos de Paula
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
Beneficiário:Antonio Claudio Tedesco
Linha de fomento: Auxílio à Pesquisa - Temático