Comparative metatranscriptomic analysis of anaerobic digesters treating anionic surfactant contaminated wastewater

Autor(es)

Midoux, Cedric ^[3]

Afiliação do(s) autor(es)

^[1] Irstea, UR HBAN, F-92761 Antony - France

• Tipo de documento: Artigo Científico
• Fonte: Science of The Total Environment; v. 649, p. 482-494, FEB 1 2019.
• Citações Web of Science: 1

Resumo

Three distinct biological reactors fed with synthetic medium (UASB_Control), synthetic medium and linear alkylbenzene sulfonate (LAS; UASB_SL), and real laundry wastewater (UASB_LW) were compared using a metatranscriptomic approach to determine putative bioindicator genes and taxonomies associated to all steps of anaerobic LAS biodegradation pathway. A homemade bioinformatics pipeline combined with an R workflow was developed to perform the RNAseq data analysis. UASB_SL and UASB_LW showed similar values of LAS biological degradation (-47%) and removal (53-55%). Rarefaction analysis revealed that 1-2 million reads were sufficient to access the whole functional capacity. In the first step of LAS biodegradation pathway, fumarate reductase subunit C was detected and taxonomically assigned to the genus Syntrophobacter (0.002% - UASB_SL; 0.0015% - UASB_LW; not detected - UASB_Control). In the second step, many enzymes related to beta-oxidation were observed and most of them with low relative abundance in UASB Control and taxonomically related with Smithella. Acinetobacter and Syntrophorhabdus. For the ring cleavage step, the abundance of 6 OCI-I CoA hydrolase putative gene was ten times higher in UASB_SL and UASB_LW when compared to UASB_Control, and assigned to Desulfomonile and Syntrophorhabdus. Finally, the adenylylsulfate reductase, taxonomically related with Desulfovibrio and Desulfomonile, was observed in the desulfonation step with the highest relative abundance in UASB_LW. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/16426-0 - Metagenômica da microbiota de reatores anaeróbios aplicados na degradação de surfactante aniônico em água residuária de lavanderia comercial
• Beneficiário: Tiago Palladino Delforno
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/00817-8 - Mineração de meta-omic dados de bioprocessos da digestão anaeróbia
• Beneficiário: Tiago Palladino Delforno
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado