In Vitro Osteogenesis Stimulation via Nano-Hydroxyapatite/Carbon Nanotube Thin Films on Biomedical Stainless Steel

Autor(es)

Ricci, Ritchelli ^[3] ; Lobo, Anderson Oliveira ^[2] ; Marciano, Fernanda Roberta ^[2]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Inst Pesquisa Desenvolvimento, Ave Shishima Hifumi 2911, BR-12244000 Sao Paulo - Brazil

^[2] Univ Brasil, Inst Ciencia & Tecnol, Rua Carolina da Fonseca 584, BR-08230030 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS; v. 11, n. 9 SEP 2018.
• Citações Web of Science: 2

Resumo

We evaluated the electrophoretic deposition of nanohydroxyapatite/superhydrop hilic multiwalled carbon nanotube composites (nHAp/MWCNT) onto stainless steel biomedical alloys for applications in bone tissue engineering. First, nHAp/MWCNT composites were dispersed into 0.042 mol.L-1 of Ca(NO3)(2)center dot 4H(2)O + 0.025 mol.L-1 NH4H2PO4 electrolytes (pH = 4.8) at two different concentrations. Next, a voltage of -2 V was applied using 316L stainless steel as a working electrode (0.27 cm(2)), a high-purity platinum coil wire was used as the auxiliary electrode, and an Ag/AgCl (3 M) electrode was used as the reference electrode. The nHAp/MWCNT composites were characterized by transmission electron microscopy. The deposited nHAp and nHAp/MWCNT films were characterized by profilometry, scanning electron microscopy, X-ray diffractometry and Raman spectroscopy. Human osteoblast cells were cultivated with the different materials and in vitro cytotoxicity was evaluated using lactate dehydrogenase (LDH) assay. The osteogenesis process was evaluated by mRNA levels of the three genes that are directly related to bone repair: Alkaline Phosphatase, Osteopontin and Osteocalcin. We showed that rough, crystalline apatite thin films containing phases of nHAp were successfully deposited onto 316L stainless steel alloys. Also, we noticed that nHAp/MWCNT thin films deposited onto 316L stainless steel alloys upregulated the expression of important genes related to bone mineralization and maturation. Our results strongly support the possibility of this new alternative to modify the surface of metallic biomedical alloys to promote bone tissue regeneration. (AU)

• Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
• Beneficiário: Fernanda Roberta Marciano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/12383-5 - Estimulação da via osteogênica in vitro de filmes finos de nano-hidroxiapatita / nanotubos de carbono em aço inoxidável biomédico
• Beneficiário: Fernanda Roberta Marciano
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
• Beneficiário: Anderson de Oliveira Lobo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores