Effects of methylene blue-mediated photodynamic therapy on a mouse model of squamous cell carcinoma and normal skin

Autor(es):Mostrar menos -

Silva, Ana Paula ^[1] ; Neves, Camila Lima ^[1] ; Silva, Elizangela dos Anjos ^[2] ; Lima Portela, Tania Cristina ^[1] ; Iunes, Renata Stecca ^[1] ; Cogliati, Bruno ^[3] ; Severino, Divinomar ^[4] ; Baptista, Mauricio da Silva ^[4] ; Zaidan Dagli, Maria Lucia ^[3] ; Hernandez Blazquez, Francisco Javier ^[5] ; Machado Cunha da Silva, Jose Roberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Cell Biol & Dev, Biomed Sci Inst, Av Prof Lineu Prestes 1524, Cidade Univ, BR-05508900 Sao Paulo, SP - Brazil

^[2] Fed Univ Pampa, Dept Med, BR 472, Km 585, BR-97501970 Uruguaiana, RS - Brazil

^[3] Univ Sao Paulo, Coll Vet Med & Zootechny, Dept Pathol, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Chem Inst, Dept Biochem, Av Prof Lineu Prestes 748, Cidade Univ, BR-05513970 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Coll Vet Med & Zootechny, Dept Surg, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 23, p. 154-164, SEP 2018.
• Citações Web of Science: 1

Resumo

Background: Photodynamic therapy is used to treat a variety of cancers and skin diseases by inducing apoptosis, necrosis, immune system activation, and/or vascular damage. Here, we describe the effects of a single photodynamic therapy session using methylene blue on a mouse model of squamous cell carcinoma and normal skin. Methods: The photodynamic therapy protocol comprised application of a 1% methylene blue solution, followed by irradiation with a diode laser for 15 min at 74 mW/cm(2), for a total dose of 100 J/cm(2). Morphological changes, cell proliferation, apoptosis, collagen quantity, immune system activity, and blood vessel number were analyzed 24 h and 15 days after photodynamic therapy. Results: In the squamous cell carcinoma group, photodynamic therapy reduced tumor size and cell proliferation and raised cytokine levels. In normal skin, it decreased cell proliferation and collagen quantity and increased apoptosis and blood vessel numbers. Conclusions: The effects of photodynamic therapy were greater on normal skin than squamous cell carcinoma tissues. The reduced epithelial thickness and keratinization of the former are factors that contribute to the efficacy of this treatment. Adjustments to the treatment protocol are necessary to potentiate the effects for squamous cell carcinoma therapy. (AU)

• Processo FAPESP: 09/51336-3 - Avaliacao histopatologica do tratamento do carcinoma espinocelular cutaneo em camundongos usando terapia fotodinamica mediada por azul de metileno
• Beneficiário: Ana Paula da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/19752-7 - Avaliação da resposta imune celular após o tratamento do carcinoma espinocelular cutâneo em camundongos usando terapia fotodinâmica
• Beneficiário: Camila Lima Neves
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/52675-3 - Avaliação histopatolotica do tratamento do carcinoma espinocelular cutâneo em camundongos usando terapia fotodinâmica mediada por azul de metileno
• Beneficiário: José Roberto Machado Cunha da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular