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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Hydroperoxide and carboxyl groups preferential location in oxidized biomembranes experimentally determined by small angle X-ray scattering: Implications in membrane structure

Texto completo
Autor(es):
De Rosa, Raffaela [1] ; Spinozzi, Francesco [2] ; Itri, Rosangela [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Phys, Sao Paulo, SP - Brazil
[2] Polytech Univ Marche, Dept Life & Environm Sci, Ancona - Italy
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1860, n. 11, p. 2299-2307, NOV 2018.
Citações Web of Science: 3
Resumo

We report small angle X-ray scattering (SAXS) data from large unilamellar vesicles as model membranes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocoline (POPC) and two oxidized species, namely its hydroperoxidized form POPC-OOH and 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC) lipid that has a carboxyl group at the end of its truncated sn-2 chain. The replacement of POPC by either POPC-OOH (POPC-OOHxPOPC1-x or PazePC (PazePC(x)POPC(1-x)), with oxidized lipid molar ratio x varying from 0.00 up to 1.00, permits to experimentally inspect changes in the membrane structural properties due to oxidation. The volume fraction distribution of each lipid chemical group along the bilayer is determined. The results quantify that 95% of the hydroperoxide group lies in the membrane polar moiety, near the carbonyl and phosphate groups, whereas just 5% of OOH group experiences the polar/apolar interface, for all values of x studied. In the case of PazePC up to x = 0.33, a bimodal distribution of the carboxyl group in the interior and polar regions of the lipid membrane is obtained, probably due to a dynamic movement of the shortened alkyl chain towards the water interface. The mean molecular area A gradually increases from 65.4 +/- 0.4 angstrom(2) for POPC bilayers to 78 +/- 2 angstrom(2) for pure POPC-OOH bilayers, whereas POPC-OOH membrane thickness resulted to be 20% thinner than the non oxidized POPC membrane. For PazePC up to x = 0.33, A increases to 67 +/- 2 angstrom(2) with 10% of membrane thinning. The SAXS results thus demonstrate how the lipid oxidation progress affects the membrane structural features, thus paving the way to better understand membrane damage under oxidative stress. (AU)

Processo FAPESP: 12/50680-5 - Fotossensibilização nas ciências da vida
Beneficiário:Mauricio da Silva Baptista
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/02511-5 - Danos físicos em membranas por processos de fotossensibilização
Beneficiário:Raffaela de Rosa
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 16/25847-4 - Estudos estruturais de dispersões lipídicas por técnicas de espalhamento de RX: avanços na análise de dados via programa Genfit
Beneficiário:Rosangela Itri
Linha de fomento: Auxílio à Pesquisa - Pesquisador Visitante - Internacional