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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genomic prediction ability for beef fatty acid profile in Nelore cattle using different pseudo-phenotypes

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Autor(es):
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Justino Chiaia, Hermenegildo Lucas [1] ; Peripolli, Elisa [1] ; de Oliveira Silva, Rafael Medeiros [1] ; Braga Feitosa, Fabiele Loise [1] ; Antunes de Lemos, Marcos Vinicius [1] ; Berton, Mariana Piatto [1] ; Olivieri, Bianca Ferreira [1] ; Espigolan, Rafael [1] ; Tonussi, Rafael Lara [1] ; Mansan Gordo, Daniel Gustavo [1] ; de Albuquerque, Lucia Galvao [1] ; de Oliveira, Henrique Nunes [1] ; Ferrinho, Adrielle Mathias [2] ; Mueller, Lenise Freitas [3] ; Kluska, Sabrina [1] ; Tonhati, Humberto [1] ; Cravo Pereira, Angelica Simone [2] ; Aguilar, Ignacio [4] ; Baldi, Fernando [1]
Número total de Autores: 19
Afiliação do(s) autor(es):
[1] UNESP, Fac Ciencias Agr & Vet, BR-14884000 Jaboticabal, SP - Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, BR-13635900 Pirassununga, SP - Brazil
[3] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, BR-13635900 Pirassununga, SP - Brazil
[4] Inst Nacl Invest Agr, Montevideo - Uruguay
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF APPLIED GENETICS; v. 59, n. 4, p. 493-501, NOV 2018.
Citações Web of Science: 0
Resumo

The aim of the present study was to compare the predictive ability of SNP-BLUP model using different pseudo-phenotypes such as phenotype adjusted for fixed effects, estimated breeding value, and genomic estimated breeding value, using simulated and real data for beef FA profile of Nelore cattle finished in feedlot. A pedigree with phenotypes and genotypes of 10,000 animals were simulated, considering 50% of multiple sires in the pedigree. Regarding to phenotypes, two traits were simulated, one with high heritability (0.58), another with low heritability (0.13). Ten replicates were performed for each trait and results were averaged among replicates. A historical population was created from generation zero to 2020, with a constant size of 2000 animals (from generation zero to 1000) to produce different levels of linkage disequilibrium (LD). Therefore, there was a gradual reduction in the number of animals (from 2000 to 600), producing a ``bottleneck effect{''} and consequently, genetic drift and LD starting in the generation 1001 to 2020. A total of 335,000 markers (with MAF greater or equal to 0.02) and 1000 QTL were randomly selected from the last generation of the historical population to generate genotypic data for the test population. The phenotypes were computed as the sum of the QTL effects and an error term sampled from a normal distribution with zero mean and variance equal to 0.88. For simulated data, 4000 animals of the generations 7, 8, and 9 (with genotype and phenotype) were used as training population, and 1000 animals of the last generation (10) were used as validation population. A total of 937 Nelore bulls with phenotype for fatty acid profiles (Sum of saturated, monounsaturated, omega 3, omega 6, ratio of polyunsaturated and saturated and polyunsaturated fatty acid profile) were genotyped using the Illumina BovineHD BeadChip (Illumina, San Diego, CA) with 777,962 SNP. To compare the accuracy and bias of direct genomic value (DGV) for different pseudo-phenotypes, the correlation between true breeding value (TBV) or DGV with pseudo-phenotypes and linear regression coefficient of the pseudo-phenotypes on TBV for simulated data or DGV for real data, respectively. For simulated data, the correlations between DGV and TBV for high heritability traits were higher than obtained with low heritability traits. For simulated and real data, the prediction ability was higher for GEBV than for Yc and EBV. For simulated data, the regression coefficient estimates (b((Yc,DGV))), were on average lower than 1 for high and low heritability traits, being inflated. The results were more biased for Yc and EBV than for GEBV. For real data, the GEBV displayed less biased results compared to Yc and EBV for SFA, MUFA, n-3, n-6, and PUFA/SFA. Despite the less biased results for PUFA using the EBV as pseudo-phenotype, the (b((Yi,DGV))) estimates obtained for the different pseudo-phenotypes (Yc, EBV and GEBV) were very close. Genomic information can assist in improving beef fatty acid profile in Zebu cattle, since the use of genomic information yielded genomic values for fatty acid profile with accuracies ranging from low to moderate. Considering both simulated and real data, the ssGBLUP model is an appropriate alternative to obtain more reliable and less biased GEBVs as pseudo-phenotype in situations of missing pedigree, due to high proportion of multiple sires, being more adequate than EBV and Yc to predict direct genomic value for beef fatty acid profile. (AU)

Processo FAPESP: 11/21241-0 - Estudo da variabilidade genética do perfil de ácidos graxos da carne de bovinos da raça Nelore terminados em confinamento
Beneficiário:Fernando Sebastián Baldi Rey
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 09/16118-5 - Ferramentas genômicas no melhoramento genético de características de importância econômica direta em bovinos da raça Nelore
Beneficiário:Lucia Galvão de Albuquerque
Linha de fomento: Auxílio à Pesquisa - Temático