The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14

Autor(es)

Martins Oliveira-Brito, Patricia Kellen ^[1] ; Roque-Barreira, Maria Cristina ^[1] ; da Silva, Thiago Aparecido ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biol Celular & Mol & Bioagentes Patogen, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 23, n. 9 SEP 2018.
• Citações Web of Science: 0

Resumo

ArtinM, a D-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing Toll-like receptor (TLR) 2 and cluster of differentiation (CD) 14 N-glycans, induces cytokine production, and promotes type 1 T helper (Th1) immunity, a process that plays an assisting role in the combat against fungal infections. We recently demonstrated that ArtinM stimulates CD4(+) T cells to produce interleukin (IL)-17 through direct interaction with CD3. Here, we further investigated the effects of ArtinM on the production of IL-17 by B cell activation. We showed that ArtinM activates murine B cells, increasing IL-17 and IL-12p40 production. The direct effect of ArtinM was sufficient to induce IL-17 production in B cells, and we did not find differences in the levels of IL-17 between the B cells purified from the wild-type (WT) and knockout (KO) mice for TLR2 or CD14 in the presence of ArtinM. Thus, the effects of ArtinM on splenic B cells through carbohydrate recognition may contribute to Th17 immunity; however, the mechanism involved is not associated with the interaction of ArtinM with TLR2 and CD14. The current work represents a pioneering effort in the understanding of the induction of IL-17 by lectins in B cells. (AU)

• Processo FAPESP: 16/23112-7 - A atividade imunomoduladora de ArtinM sobre o curso da infecção por Cryptococcus gattii
• Beneficiário: Patrícia Kellen Martins Oliveira Brito
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/04877-2 - Delineamento de novas estratégias terapêuticas, baseadas em reconhecimento de carboidratos, contra a criptococose
• Beneficiário: Thiago Aparecido da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/10446-4 - Mecanismos da imunomodulação por ArtinM: bases para o desenvolvimento de nova terapia anti-fúngica
• Beneficiário: Maria Cristina Roque Antunes Barreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular