Use of a Non-Crosslinked Collagen Membrane During Guided Bone Regeneration Does Not Interfere With the Bone Regenerative Capacity of the Periosteum

Autor(es)

Pinotti, Felipe Eduardo ^[1] ; Pimentel Lopes de Oliveira, Guilherme Jose ^[2] ; Scardueli, Cassio Rocha ^[1] ; de Medeiros, Marcell Costa ^[1] ; Stavropoulos, Andreas ^[3] ; Chierici Marcantonio, Rosemary Adriana ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Diag & Surg, 1680 Humaita St, BR-14801130 Araraquara, SP - Brazil

^[2] Univ Fed Uberlandia, Dept Periodontol, Uberlandia, MG - Brazil

^[3] Malmo Univ, Fac Odontol, Dept Periodontol, Malmo - Sweden

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY; v. 76, n. 11 NOV 2018.
• Citações Web of Science: 1

Resumo

Purpose: To assess whether the use of a non-crosslinked porcine collagen type I and III bi-layered membrane inter-positioned between the periosteum and a bone defect would interfere with the bone regenerative capacity of the periosteum. Materials and Methods: Sixty rats, each with 1 critical-size calvarial defect (CSD; diameter, 5 mm) in the parietal bone, were randomly allocated to 1 of 3 equal-size groups after CSD creation: 1) the periosteum was excised and the flap was repositioned without interposition of a membrane (no-periosteum {[}NP] group); 2) the flap including the periosteum was repositioned (periosteum {[}P] group); and 3) a non-crosslinked collagen membrane was inter-positioned between the flap, including the periosteum, and the bone defect (membrane {[}M] group). Micro-computed tomography, qualitative histology, immunohistochemistry, and reverse transcription real-time quantitative polymerase chain reaction were performed at 3, 7, 15, and 30 days postoperatively. Results: A markedly increased radiographic residual defect length was observed in the NP group compared with the P group at 30 days. The NP group also presented a smaller radiographic bone fill area than the P group at 15 and 30 days and then the M group at 30 days. The P and M groups exhibited considerably greater expression of bone morphogenetic protein-2 and osteocalcin than the NP group at 7 days; expression of transforming growth factor-beta 1 was considerably greater in the NP group at 15 days. Further, the P group presented considerably higher gene expression levels of Runx2 and Jagged1 at 7 days and of alkaline phosphatase at 3 and 15 days compared with the M and NP groups. Conclusion: Interposition of this specific non-crosslinked collagen membrane between the periosteum and the bone defect during guided bone regeneration interferes only slightly, if at all, with the bone regenerative capacity of the periosteum. (C) 2018 American Association of Oral and Maxillofacial Surgeons (AU)

• Processo FAPESP: 13/21560-4 - Influência da readaptação do periósteo sobre o reparo em defeitos críticos de calotas em ratos
• Beneficiário: Felipe Eduardo Pinotti
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica