Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Milk fat protects Bifidobacterium animalis subsp lactis Bb-12 from in vitro gastrointestinal stress in potentially synbiotic table spreads

Texto completo
Autor(es):
dos Santos, Clara Simone [1] ; Batista de Souza, Cinthia Hoch [1, 2] ; Padilha, Marina [3, 1] ; Gioielli, Luiz Antonio [1] ; Rodrigues Ract, Juliana Neves [1] ; Saad, Susana Marta Isay [3, 1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Av Prof Lineu Prestes 580, B16, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ North Parana, Dairy Sci & Technol, Rua Marselha 591, BR-86041140 Londrina, PR - Brazil
[3] Univ Sao Paulo, Food Res Ctr, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: FOOD & FUNCTION; v. 9, n. 8, p. 4274-4281, AUG 1 2018.
Citações Web of Science: 2
Resumo

The viability and the in vitro gastrointestinal survival of Bifidobacterium animalis subsp. lactis Bb-12 (Bifidobacterium Bb-12) in table spreads with different proportions of milk fat (MF) and palm olein (PO) (MF : PO 40 : 60 and MF : PO 20 : 80) were investigated for up to 28 days of storage at 5 degrees C. Moreover, qPCR alone and combined with propidium monoazide (PMA) were compared with the traditional plate count method for determining the in vitro gastrointestinal survival of Bifidobacterium Bb-12 in table spreads after 35 days of storage. Formulations showed probiotic viabilities ranging from 8 to 9 log CFU g(-1) across the whole storage period, and the milk fat and palm olein in different concentrations did not affect this viability. Bifidobacterium Bb-12 showed good survival after six hours under in vitro simulated gastrointestinal conditions during the studied storage period, with average reductions of 1.70 (MF : PO 40 : 60) and 2.16 log CFU g(-1) (MF : PO 20 : 80). The results of the qPCR with PMA treatment and the plate count method were similar and the qPCR without PMA treatment was shown to overestimate the Bifidobacterium Bb-12 populations. However, the MF : PO 40 : 60 spread showed a Bb-12 population between 0.76 and 1.43 log CFU g(-1) higher than that of MF : PO 20 : 80. Thus, the results showed that table spreads, especially food matrices with a higher proportion of milk fat, are suitable for the incorporation of Bifidobacterium Bb-12. (AU)

Processo FAPESP: 14/04784-9 - Aprimoramento das características sensoriais de margarina probiótica e avaliação de seu potencial funcional
Beneficiário:Clara Simone dos Santos
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
Beneficiário:Bernadette Dora Gombossy de Melo Franco
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 15/15863-0 - Aplicação de lipídios estruturados na obtenção de spread como alimento funcional probiótico e prebiótico
Beneficiário:Juliana Neves Rodrigues Ract
Linha de fomento: Auxílio à Pesquisa - Regular