Peritumoural adipose tissue pro-inflammatory cytokines are associated with tumoural growth factors in cancer cachexia patients

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Pinto Neto, Nelson Inacio ^[1] ; de Pinho Murari, Ariene Soares ^[2] ; Oyama, Lila Missae ^[1] ; Otoch, Jose Pinhata ^[2] ; Martins Alcantara, Paulo Sergio ^[2] ; Tokeshi, Flavio ^[2] ; Figueredo, Raquel Galvao ^[3] ; Alves, Michele Joana ^[3] ; Carola Correia Lima, Joanna Darck ^[3] ; de Matos-Neto, Emidio Marques ^[4] ; Seelaender, Marilia ^{[3, 2]} ; Oller do Nascimento, Claudia Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Fisiol, Escola Paulista Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Clin Surg, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Canc Metab Res Grp, Sao Paulo - Brazil

^[4] Univ Fed Piaui, Dept Phys Educ, Piaui - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE; v. 9, n. 6, p. 1101-1108, DEC 2018.
• Citações Web of Science: 4

Resumo

Background Cancer cachexia (CC) is a multifactorial syndrome, often irreversible, that affects patients with cancer influenced, in part, by the inflammatory condition. Peritumoural adipose tissue produces adipokines and angiogenic, apoptotic, and growth factors; given the possible crosstalk between the peritumoural adipose tissue and tumour, these may play an important role in cancer biology and carcinogenesis. Methods Results The aim of this study was to evaluate the factors produced by peritumoural adipose tissue in a cohort of 16 colorectal cancer patients with either weight-stable cancer (WSC; n = 7) or CC (n = 9). The study was approved by the Ethics Research Committee (972.914). Samples of peritumoural adipose tissue were analysed for concentrations of TNF-alpha, IL-1 beta, STAT-1, STAT-3, RANTES, IL-1Ra, IP-10, IL-15, MCP-1, IFN-alpha, GCSF, FADD, and TGF-beta. The cytokines and proteins were measured using Multiplex. Correlations between the proteins and cytokines were evaluated. TNF-alpha, STAT-1, and FADD, a factor involved in apoptosis, were significantly higher in CC group than in the WSC group. In the peritumoural adipose tissue of the CC group, RANTES showed a significant positive correlation with IL-1Ra and IP-10 and a negative correlation with IFN-alpha; and GCSF showed significant negative correlations with IL-1Ra, IP-10, IL-15, and MCP-1 and a positive correlation with IFN-alpha. In the peritumoural adipose tissue of the WSC group, no significant correlations were detected between RANTES, GCSF, IL-3, FADD, and STAT-1 and the cytokines/chemokines analysed. Conclusions These results indicated that inflammatory and tumorigenic pathways were altered in peritumoural adipose tissue in CC. Furthermore, inflammatory cytokines were correlated with growth factors in the peritumoural adipose tissue of cachectic patients, suggesting that inflammatory cytokines modulated the proliferative environment closely linked to the tumour. (AU)

• Processo FAPESP: 12/50079-0 - Inflamação sistêmica em pacientes com caquexia associada ao câncer: mecanismos e estratégias terapêuticas, uma abordagem em medicina translacional
• Beneficiário: Marilia Cerqueira Leite Seelaender
• Modalidade de apoio: Auxílio à Pesquisa - Temático