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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Maternal immunization downregulates offspring TCD4 regulatory cells (Tregs) thymic maturation without implications for allergy inhibition

Texto completo
Autor(es):
de Oliveira, Marilia Garcia [1] ; de Lima Lira, Aline Aparecida [1] ; Sgnotto, Fabio da Ressureicao [2] ; Sabo Inoue, Amanda Harumi [1] ; Beltrame, Giovanna Rossi [1] ; da Silva, Debora [3] ; Menghini, Ricardo Palamar [3] ; da Silva Duarte, Alberto Jose [1, 4] ; Victor, Jefferson Russo [3, 1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Div Dermatol, Lab Med Invest LIM 56, Med Sch, Sao Paulo - Brazil
[2] Univ Sao Paulo, Div Hematol, Med Sch, Sao Paulo - Brazil
[3] Laureate Int Univ, Div Environm Hlth, FMU, Sao Paulo - Brazil
[4] Univ Sao Paulo, Div Pathol, Med Sch, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Scandinavian Journal of Immunology; v. 88, n. 6 DEC 2018.
Citações Web of Science: 1
Resumo

The regulation of offspring allergy development mediated by maternal immunization was evidenced by several groups, and this mechanism seems to involve the induction of regulatory T cells (Tregs) on offspring. Here, we aimed to evaluate whether the effect of maternal immunization on offspring Tregs occurs as a result of peripheral or central modulation. Briefly, C57BL/6 female mice were immunized with OVA in Alum or Alum alone and boosted with OVA in saline or saline only after 10 and 20 days. Non-immunized offspring serum, thymus and spleen were evaluated at 3 or 20 days old, and some groups of pups were submitted to neonatal OVA-immunization protocol for the subsequent evaluation of antibody production and allergic response. Our experimental protocol could be validated because maternal OVA-immunization inhibited offspring allergic response as evidenced by the suppression of offspring IgE production and allergic lung inflammation. Interestingly, maternal immunization reduced the frequency of offspring thymic Tregs with an opposite effect on spleen Tregs. Furthermore, after neonatal immunization, the frequency of lung-infiltrated Tregs was also augmented on offspring from immunized mothers. In conclusion, maternal OVA-immunization can inhibit the thymic maturation of offspring Tregs without implications on peripheral Tregs induction and allergy inhibition. (AU)

Processo FAPESP: 18/05181-7 - Avaliação translacional do efeito modulador e/ou regulador dos anticorpos IgG na maturação de linfócitos TCD4, TCD8, nTreg, Tgammadelta e B intra-tímicos de neonatos.
Beneficiário:Alberto José da Silva Duarte
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/18558-9 - Avaliação in vitro da influência do repertório IgG murino sobre a maturação de linfócitos B e Tgamma-delta com potencial regulador e/ou modulador
Beneficiário:Amanda Harumi Sabô Inoue
Linha de fomento: Bolsas no Brasil - Iniciação Científica