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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In vitro antibacterial and cytotoxic activities of carvacrol and terpinen-4-ol against biofilm formation on titanium implant surfaces

Texto completo
Autor(es):
Maquera-Huacho, Patricia Milagros [1] ; Tonon, Caroline Coradi [1] ; Correia, Marilia Ferreira [2] ; Francisconi, Renata Serignoli [2] ; Ferreira Bordini, Ester Alves [3] ; Marcantonio, Elcio [1] ; Palomari Spolidorio, Denise Madalena [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Oral Diag & Surg, Araraquara, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil
[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BIOFOULING; v. 34, n. 6, p. 699-709, 2018.
Citações Web of Science: 1
Resumo

This study evaluated the antibacterial properties of carvacrol and terpinen-4-ol against Porphyromonas gingivalis and Fusobacterium nucleatum and its cytotoxic effects on fibroblast cells. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were examined. The minimum biofilm inhibition concentration (MBIC) was evaluated by XTT assay. Biofilm decontamination on titanium surfaces was quantified (CFU ml(-1)), evaluated by confocal laser scanning microscopy (CLSM) and cytotoxic activity by MTT. The MIC and MBC for carvacrol were 0.007% and 0.002% for P. gingivalis and F. nucleatum, and 0.06% for terpinen-4-ol for both microorganisms. The MBIC for carvacrol was 0.03% and 0.06% for P. gingivalis and F. nucleatum, and for terpinen-4-ol was 0.06% and 0.24%. The results indicated anti-biofilm activity using carvacrol (0.26%, 0.06%) and terpinen-4-ol (0.95%, 0.24%) and showed cytotoxic activity similar to chlorohexidine (CHX). However, terpinen-4-ol (0.24%) showed higher cell viability than other treatments. Carvacrol and terpinen-4-ol showed antibacterial activity in respect of reducing biofilms. Moreover, CHX-like cytotoxicity was observed. (AU)

Processo FAPESP: 09/54035-4 - EMU: facility para estudos avançados de materiais nanoestruturados e biossistemas / FAMA
Beneficiário:Igor Polikarpov
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 15/08742-1 - Caracterização do terpinen-4-ol sobre fungos e bactérias: avaliação do efeito sinérgico/aditivo, citotoxicidade, adesão em co-cultura e alteração dos fatores de virulência
Beneficiário:Denise Madalena Palomari Spolidorio
Linha de fomento: Auxílio à Pesquisa - Regular