Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies

Texto completo
Autor(es):
de Queiroz, Joao Vitor [1] ; Souza Vieira, Jose Cavalcante [2] ; de Oliveira, Grasieli [2] ; Braga, Camila Pereira [3] ; Bataglioli, Izabela da Cunha [1] ; da Silva, Janaina Macedo [2] ; de Paula Araujo, Wellington Luiz [1] ; Padilha, Pedro de Magalhaes [2, 1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu, SP - Brazil
[3] Univ Nebraska, Dept Biochem, Lincoln, NE 68583 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 187, n. 1, p. 291-300, JAN 2019.
Citações Web of Science: 3
Resumo

Predator fish can accumulate high levels of mercury, which qualifies them as potential indicators of this toxic metal. The predatory species Brachyplatystoma filamentosum, popularly known as filhote, is among the most consumed species in the Brazilian Amazon. Continuing the metalloproteomic studies of mercury in Amazonian fishes that have been developed in the last 5years, the present paper provides the data of protein characterization associated with mercury in muscle and liver samples of filhote (Brachyplatystoma filamentosum) collected in the Madeira River, Brazilian Amazon. The mercury concentration in the muscle and liver samples was determined by graphite furnace atomic absorption spectrometry (GFAAS). The protein fraction was extracted in an aqueous medium, and later, a fractional precipitation procedure was performed to obtain the protein pellets. Then, the proteome of the tissue samples of this fish species was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and a mercury mapping of the protein spots was carried out by GFAAS after acid digestion. Protein spots that had mercury were characterized by mass spectrometry with electrospray ionization in sequence (ESI-MS/MS) after tryptic digestion. It was possible to characterize 11 mercury-associated protein spots that presented biomarker characteristics and could be used to monitor mercury in fish species of the Amazon region. Thus, the metalloproteomic strategies used in the present study allowed us to characterize 11 mercury-associated protein spots. It should be noted that the protein spots identified as GFRP, TMEM186, TMEM57B, and BHMT, which have coordination sites for elements with characteristics of soft acids, such as mercury, can be used as biomarkers of mercury contamination in monitoring studies of this toxic metal in fish species from the Amazon region. (AU)

Processo FAPESP: 16/19404-2 - Caracterização de biomarcadores de toxicidade de mercúrio em peixes da Bacia do Rio Madeira-Rondônia utilizando-se estratégias metaloproteômicas
Beneficiário:Pedro de Magalhães Padilha
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/21297-1 - Metalotioneínas em peixes da região amazônica: estudo metalômico associado ao mercúrio
Beneficiário:Pedro de Magalhães Padilha
Linha de fomento: Auxílio à Pesquisa - Regular