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Evaluation of some effects on plant metabolism through proteins and enzymes in transgenic and non-transgenic soybeans after cultivation with silver nanoparticles

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Autor(es):
Galazzi, Rodrigo Moretto [1, 2] ; Lopes Junior, Cicero Alves [1, 2] ; de Lima, Tatiani Breneli [3] ; Gozzo, Fabio Cesar [3] ; Zezzi Arruda, Marco Aurelio [1, 2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Chem, Spectrometry Sample Preparat & Mechanizat Grp, UNICAMP, POB 6154, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Natl Inst Sci & Technol Bioanalyt, Inst Chem, UNICAMP, POB 6154, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Chem, Dalton Mass Spectrometry Grp, POB 6154, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PROTEOMICS; v. 191, n. SI, p. 88-106, JAN 16 2019.
Citações Web of Science: 1
Resumo

To evaluate the effects of silver nanoparticles (AgNP) exposition, transgenic (through gene cp4EPSPS) and non-isogenic non-transgenic soybeans were cultivated in the presence or absence of AgNP or silver nitrate (AgNO3) at 50 mg/kg of silver. Physiological aspects of the plants including mass production and development of roots, proteomics such as protein amount and differential proteins, enzymes and lipid peroxidation were determined after exposition. The mass production of non-transgenic plants treated with AgNP or AgNO3 was decreased by 25 and 19%, respectively, on their mass based, while for transgenic soybean this effect was observed for AgNP cultivation only. Fifty-nine proteins were identified from the differentially abundant spots by two-dimensional difference gel electrophoresis and nano-electrospray ionization liquid chromatography coupled tandem mass spectrometry. Identified species as ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), ATP synthase, superoxide dismutase (SOD), related to plant metabolism were less abundant for the cultivation with either AgNP and AgNO3 than the control. Finally, this work demonstrated significant correlation as evidenced by changes in lipid peroxidation content and catalase activity, which were a result of exposure to either AgNP or AgNO3 cultivations. Further, necrotic areas in the basal part of the stems and damage or chlorotic areas were found in the leaves. Significance: Once nanoparticles have been employed for several applications in recent years and they can be released in the environmental matrices, this study highlights proteomic and enzymatic alterations in transgenic and non-transgenic soybeans, an important crop, after cultivation with silver nanoparticles. Such strategy employing proteomic and enzymatic approaches to evaluate soybeans exposed to silver nanoparticles has not yet been reported. Therefore, the results obtained in this study can expand the information concerning the effects of silver nanoparticles in soybean plants. (AU)

Processo FAPESP: 13/15646-3 - Avaliação de plantas de soja transgênica e não-transgênica cultivadas em solo tratado com nanopartículas de prata
Beneficiário:Rodrigo Moretto Galazzi
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 16/07384-7 - Bioimageamento elementar por meio da ablação a laser e espectrometria de massas com plasma indutivamente acoplado
Beneficiário:Marco Aurelio Zezzi Arruda
Linha de fomento: Auxílio à Pesquisa - Regular