Global proteomic and functional analysis of Crotalus durissus collilineatus individual venom variation and its impact on envenoming

Autor(es):Mostrar menos -

de Oliveira, Isadora Sousa ^[1] ; Cardoso, Iara Aime ^[1] ; Figueiredo Bordon, Karla de Castro ^[1] ; Imai Carone, Sante Emmanuel ^[2] ; Boldrini-Franca, Johara ^[1] ; Pucca, Manuela Berto ^[3] ; Zoccal, Karma Furlani ^[2] ; Faccioli, Lucia Helena ^[2] ; Sampaio, Suely Vilela ^[2] ; Rosa, Jose Cesar ^{[4, 5]} ; Arantes, Eliane Candiani ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto, SP - Brazil

^[3] Univ Fed Roraima, Sch Med, Hlth Sci Ctr, Boa Vista, RR - Brazil

^[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Prot Chem Ctr, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Mol & Cell Biol & Pathogen Bioagents, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOMICS; v. 191, n. SI, p. 153-165, JAN 16 2019.
• Citações Web of Science: 5

Resumo

Individual variations studies are important to understand the snakebite envenoming and to improve the anti-venom production and its effectiveness. In this way, the objective of this study was a comparative analysis of intraspecific variation in the venom composition of 22 Crotalus durissus collilineatus specimens through proteomic techniques. Venoms were fractionated by RP-FPLC, and analyzed by SDS-PAGE and mass spectrometry. Although similar, chromatographic and electrophoretic profiles showed significant qualitative and quantitative differences. Some venom components were identified for the very first time in C. d collilineatus, such as glutathione peroxidase, nerve growth factor, 5'-nucleotidase, angiotensin-converting enzyme, carboxypeptidase, phosphodiesterase, glutaminyl cyclase and phospholipase B. Regarding hyaluronidase activity, 2 venoms did not present detectable enzyme activity in the tested amounts. Additionally, in vivo crotalic envenoming in mice showed that venoms from different specimens resulted in diversified changes of biochemical and immunological parameters, such as creatine kinase and interleukin 6. This study demonstrated significant intraspecific variations in the venom of C. d. collilineatus, which may impact the production and effectiveness of the antivenom therapy. Biological significance: This study performed the proteomic and functional analyzes of 22 C. d. collilineatus individual venoms and verified the occurrence of quali and quantitative variations among them. The venoms evaluated caused envenomings with different changes in biochemical and immunological parameters. These results confirm the need to use a pool of venoms with the greatest possible variability in the preparation of antivenoms, in order to improve their effectiveness. In addition, this study was able to identify for the first time 8 different proteins in this subspecies venom, increasing knowledge about its composition and showing that it is a source of these proteins with possible biotechnological applications. (AU)

• Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
• Beneficiário: Suely Vilela
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/03332-7 - Estudo da relação entre ativação do inflamassoma e produção de mediadores lipídicos com a inflamação pulmonar induzida por veneno de escorpião com e sem hialuronidase
• Beneficiário: Karina Furlani Zoccal
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/16182-3 - Novas perspectivas funcionais de uma serinoprotease de Crotalus durissus collilineatus: ação sobre canais iônicos dependentes de voltagem
• Beneficiário: Johara Boldrini França
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado