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Gene Co-expression Network Reveals Potential New Genes Related to Sugarcane Bagasse Degradation in Trichoderma reesei RUT-30

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Bonin, Gustavo Pagotto [1, 2] ; Carazzolle, Marcelo Falsarella [3] ; Correa dos Santos, Renato Augusto [4] ; Riano-Pachon, Diego Mauricio [5] ; de Castro Oliveira, Juliana Velasco [1, 2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Programa Posgrad Genet & Biol Mol, UNICAMP, Campinas, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Biol, LGE, Dept Genet Evolucao Microbiol & Imunol, Campinas, SP - Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto - Brazil
[5] Univ Sao Paulo, Ctr Energia Nucl Agr, Pracicaba - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Citações Web of Science: 1

The biomass-degrading fungus Trichoderma reesei has been considered a model for cellulose degradation, and it is the primary source of the industrial enzymatic cocktails used in second-generation (2G) ethanol production. However, although various studies and advances have been conducted to understand the cellulolytic system and the transcriptional regulation of T. reesei, the whole set of genes related to lignocellulose degradation has not been completely elucidated. In this study, we inferred a weighted gene co-expression network analysis based on the transcriptome dataset of the T. reesei RUT-C30 strain aiming to identify new target genes involved in sugarcane bagasse breakdown. In total, similar to 70% of all the differentially expressed genes were found in 28 highly connected gene modules. Several cellulases, sugar transporters, and hypothetical proteins coding genes upregulated in bagasse were grouped into the same modules. Among them, a single module contained the most representative core of cellulolytic enzymes (cellobiohydrolase, endoglucanase, beta-glucosidase, and lytic polysaccharide monooxygenase). In addition, functional analysis using Gene Ontology (GO) revealed various classes of hydrolytic activity, cellulase activity, carbohydrate binding and cation:sugar symporter activity enriched in these modules. Several modules also showed GO enrichment for transcription factor activity, indicating the presence of transcriptional regulators along with the genes involved in cellulose breakdown and sugar transport as well as other genes encoding proteins with unknown functions. Highly connected genes (hubs) were also identified within each module, such as predicted transcription factors and genes encoding hypothetical proteins. In addition, various hubs contained at least one DNA binding site for the master activator Xyr1 according to our in silico analysis. The prediction of Xyr1 binding sites and the co-expression with genes encoding carbohydrate active enzymes and sugar transporters suggest a putative role of these hubs in bagasse cell wall deconstruction. Our results demonstrate a vast range of new promising targets that merit additional studies to improve the cellulolytic potential of T. reesei strains and to decrease the production costs of 2G ethanol. (AU)

Processo FAPESP: 14/11766-7 - Caracterização de novos fatores de transcrição de Trichoderma reesei envolvidos na degradação da biomassa lignocelulósica
Beneficiário:Juliana Velasco de Castro Oliveira
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
Processo FAPESP: 17/18987-7 - Construção de cepas mutantes de Trichoderma reesei e análise de elementos regulatórios para identificar e caracterizar novos genes com potencial biotecnológico na degradação de lignocelulose
Beneficiário:Gustavo Pagotto Borin
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
Processo FAPESP: 15/08222-8 - Análise da co-regulação transcricional e identificação de genes de interesse biotecnológico em Trichoderma reesei
Beneficiário:Gustavo Pagotto Borin
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 14/15799-7 - Análise genômica da levedura xilanolítica Pseudozyma brasiliensis
Beneficiário:Renato Augusto Corrêa dos Santos
Linha de fomento: Bolsas no Brasil - Mestrado