| Texto completo | |
| Autor(es): |
Moreno, Natalia Cestari
[1]
;
Machado Garcia, Camila Carriao
[2, 3]
;
Munford, Veridiana
[1]
;
Reily Rocha, Clarissa Ribeiro
[1]
;
Pelegrini, Alessandra Luiza
[1]
;
Corradi, Camila
[1]
;
Sarasin, Alain
[4]
;
Martins Menck, Carlos Frederico
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil
[2] Univ Fed Ouro Preto, Dept Biol Sci, Ouro Preto, MG - Brazil
[3] Univ Fed Ouro Preto, NUPEB, Ouro Preto, MG - Brazil
[4] Univ Paris Sud, Inst Gustave Roussy, CNRS, Lab Genet Instabil & Oncogenesis, UMR8200, Villejuif - France
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Free Radical Biology and Medicine; v. 131, p. 432-442, FEB 1 2019. |
| Citações Web of Science: | 3 |
| Resumo | |
The UVA component of sunlight induces DNA damage, which are basically responsible for skin cancer formation. Xeroderma Pigmentosum Variant (XP-V) patients are defective in the DNA polymerase pol eta that promotes translesion synthesis after sunlight-induced DNA damage, implying in a clinical phenotype of increased frequency of skin cancer. However, the role of UVA-light in the carcinogenesis of these patients is not completely understood. The goal of this work was to characterize UVA-induced DNA damage and the consequences to XP-V cells, compared to complemented cells. DNA damage were induced in both cells by UVA, but lesion removal was particularly affected in XP-V cells, possibly due to the oxidation of DNA repair proteins, as indicated by the increase of carbonylated proteins. Moreover, UVA irradiation promoted replication fork stalling and cell cycle arrest in the S-phase for XP-V cells. Interestingly, when cells were treated with the antioxidant N-acetylcysteine, all these deleterious effects were consistently reverted, revealing the role of oxidative stress in these processes. Together, these results strongly indicate the crucial role of oxidative stress in UVA-induced cytotoxicity and are of interest for the protection of XP-V patients. (AU) | |
| Processo FAPESP: | 14/15982-6 - Consequências de deficiências de reparo de lesões no genoma |
| Beneficiário: | Carlos Frederico Martins Menck |
| Linha de fomento: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 12/16929-6 - Efeito da luz UVA em células de pacientes com Xeroderma pigmentosum variante |
| Beneficiário: | Natália Cestari Moreno |
| Linha de fomento: | Bolsas no Brasil - Doutorado |