New Insights on Moojase, a Thrombin-Like Serine Protease from Bothrops moojeni Snake Venom

Autor(es)

Amorim, Fernanda G. ^[1] ; Menaldo, Danilo L. ^[1] ; Carone, Sante E. I. ^[1] ; Silva, Thiago A. ^[1] ; Sartim, Marco A. ^[1] ; De Pauw, Edwin ^[2] ; Quinton, Loic ^[2] ; Sampaio, Suely V. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Lab Toxinol, BR-14040903 Ribeirao Preto - Brazil

^[2] Univ Liege, Dept Chem, Lab Mass Spectrometry, B-4000 Liege - Belgium

• Tipo de documento: Artigo Científico
• Fonte: TOXINS; v. 10, n. 12 DEC 2018.
• Citações Web of Science: 1

Resumo

Snake venom serine proteases (SVSPs) are enzymes that are capable of interfering in various parts of the blood coagulation cascade, which makes them interesting candidates for the development of new therapeutic drugs. Herein, we isolated and characterized Moojase, a potent coagulant enzyme from Bothrops moojeni snake venom. The toxin was isolated from the crude venom using a two-step chromatographic procedure. Moojase is a glycoprotein with N-linked glycans, molecular mass of 30.3 kDa and acidic character (pI 5.80-6.88). Sequencing of Moojase indicated that it is an isoform of Batroxobin. Moojase was able to clot platelet-poor plasma and fibrinogen solutions in a dose-dependent manner, indicating thrombin-like properties. Moojase also rapidly induced the proteolysis of the A chains of human fibrinogen, followed by the degradation of the B chains after extended periods of incubation, and these effects were inhibited by PMSF, SDS and DTT, but not by benzamidine or EDTA. RP-HPLC analysis of its fibrinogenolysis confirmed the main generation of fibrinopeptide A. Moojase also induced the fibrinolysis of fibrin clots formed in vitro, and the aggregation of washed platelets, as well as significant amidolytic activity on substrates for thrombin, plasma kallikrein, factor Xia, and factor XIIa. Furthermore, thermofluor analyses and the esterase activity of Moojase demonstrated its very high stability at different pH buffers and temperatures. Thus, studies such as this for Moojase should increase knowledge on SVSPs, allowing their bioprospection as valuable prototypes in the development of new drugs, or as biotechnological tools. (AU)

• Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
• Beneficiário: Suely Vilela
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/20641-9 - Integração da abordagens ômicas para obter o perfil da peçonha da serpente Bothrops moojeni: revelando novas toxinas e divergências ontogênicas
• Beneficiário: Fernanda Gobbi Amorim
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 15/26609-7 - Venômica da serpente Bothrops moojeni: aplicação de toxinas de interesse biotecnológico obtidas por técnicas ômicas
• Beneficiário: Fernanda Gobbi Amorim
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado