Busca avançada
Ano de início
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bioconversion of crude glycerol from waste cooking oils into hydrogen by sub-tropical mixed and pure cultures

Texto completo
Rodrigues, Caroline Varella [1, 2] ; Nespeca, Maurilio Gustavo [3] ; Sakamoto, Isabel Kimiko [4] ; de Oliveira, Jose Eduardo [3] ; Amancio Varesche, Maria Bernadete [4] ; Maintinguer, Sandra Imaculada [1, 5]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] UNESP, Bioenergy Res Inst IPBEN, BR-13550230 Rio Claro, SP - Brazil
[2] UNESP, Inst Chem IQ, Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil
[3] UNESP, Inst Chem CEMPEQC, Ctr Monitoring & Res Qual Fuels Biofuels Crude Oi, Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil
[4] Univ Sao Paulo, Sch Engn Sao Carlos, Dept Hydraul & Sanitat, Ave Joao Dagnone 1100, BR-13563120 Sao Carlos, SP - Brazil
[5] Univ Araraquara, R Voluntarios Patria 1309, BR-14801320 Araraquara, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 44, n. 1, SI, p. 144-154, JAN 1 2019.
Citações Web of Science: 5

This study compared the biohydrogen generation by sub-tropical mixed and pure cultures from the crude glycerol from the biodiesel production using waste cooking oils (WCO). The crude glycerol was pretreated by pH adjustment. The mixed culture was obtained from a subtropical granular sludge of the UASB (Upflow Anaerobic Sludge Blanket) reactor used in the treatment of vinasse from sugarcane of ethanol and sugar industry. It was heat treated in order to inactivate hydrogen-consuming bacteria, which was identified by Illumina MiSeq Sequencing with a relative abundance of 97.96% Firmicutes Philum, 91.81% Clostridia Class and 91.81% Clostridiales Order. The pure culture was isolated from a sub-tropical granular sludge from UASB reactor of treating brewery wastewater and identified as Enterobacter sp. (KP893397). Two assays were carried in anaerobic batch reactors in order to verify the hydrogen production from crude glycerol bioconversion with: (I) mixed culture and (II) pure culture. The experiments were conducted at 37 degrees C, initial pH of 5.5 for assay I and 7.0 for assay II, with 20 g COD L-1 of crude glycerol. The crude glycerol consumption was 56.2% and 88.0% for the assay I and II, respectively. The hydrogen yields were 0.80 moL H-2 mol(-1) glycerol for the assay I and 0.13 moL H-2 Mol(-1) glycerol for the assay II. Enterobacter sp. preferred the reductive metabolic route, generating 1460.0 mg L-1 of 1,3-propanediol, and it showed to be more sensitive in the presence of methanol from crude glycerol than mixed culture that preferred the oxidative metabolic route with biohydrogen generation. The mixed culture was more able to generate H-2 than pure culture from the crude glycerol coming from the biodiesel production using WCO. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 17/11767-1 - AEM 2017 - 3th International Conference on Hydrogen
Beneficiário:Sandra Imaculada Maintinguer
Linha de fomento: Auxílio à Pesquisa - Reunião - Exterior