Genotoxic and cytotoxic effects of neolignans isolated from Nectandra leucantha (Lauraceae)

Autor(es)

de Sousa, Fernanda S. ^[1] ; Nunes, Emilene A. ^[2] ; Gomes, Kaio S. ^[2] ; Cerchiaro, Giselle ^[2] ; Lago, Joao Henrique G. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Inst Environm, Chem & Pharmaceut Sci, Diadema, SP - Brazil

^[2] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY IN VITRO; v. 55, p. 116-123, MAR 2019.
• Citações Web of Science: 0

Resumo

In this work was evaluated the cytotoxic activity of dehydrodieugenol B (1) and methyldehydrodieugenol B (2) isolated from Nectandra leucantha (Lauraceae) through cytokinesis-block micronucleus (CBMN) and Comet assay. Compounds 1 and 2 displayed in vitro toxicity against human melanoma cells (SKMEL-147) with IC50 values of 4.4 and 43.6 mu g/mL, respectively. The interaction of these compounds with resistant human melanoma cell line SKMEL-29 was also investigated. Obtained results showed a concentration-response relationship for DNA damage (DI and D%) in SKMEL-29 cells for compounds 1 and 2 causing an increase in DNA damage on their lower concentrations. Concerning the cytokinesis-block micronucleus cytome (CBMNCyt) assay, all treatments demonstrated an increase in cytostatic and cytotoxic indexes. In micronucleus quantification, compound 1 displayed higher index in comparison to control. Regarding necrotic and apoptotic cells, treatments with SKMEL-29 cells demonstrated 100% of cell death induced by compounds 1 and 2 at 25 and 88 mu g/mL, respectively. Additionally, it was observed that apoptosis is prevalent in SKMEL-147 cells treated with compound 1, while necrotic cells were observed in SKMEL-29 cells treated with both compounds. In conclusion, compounds 1 and 2 are suggested as promising cytotoxic agents against human melanoma resistant cells, emphasizing the potential use of these neolignans for the treatment of melanoma. (AU)

• Processo FAPESP: 15/11936-2 - Emprego da quimiodiversidade de espécies vegetais de áreas remanescentes da Mata Atlântica do Estado de São Paulo na seleção de protótipos biologicamente ativos
• Beneficiário: João Henrique Ghilardi Lago
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/24475-0 - Estudos sobre a ação de íons metálicos e da SOD1 em danos a biomoléculas em cultura de células neuronais sob estresse oxidativo e neurodegenerativo
• Beneficiário: Emilene Arusievicz Nunes
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/04143-6 - Metabólitos naturais e semissintéticos dos galhos de Nectandra leucantha Ness & Mart. (Lauraceae): caracterização molecular e avaliação do potencial e mecanismo antitumoral contra melanoma
• Beneficiário: Fernanda Samara de Sousa
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/07885-1 - Biomoléculas oriundas de espécies vegetais de áreas remanescentes da Mata Atlântica e do Cerrado para tratamento de doenças tropicais negligenciadas - aspectos químicos e farmacológicos
• Beneficiário: João Henrique Ghilardi Lago
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Regular