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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts

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Autor(es):
Flávia Teresa Hansen Pacheco ; Maria Estela Silva-Stenico ; Augusto Etchegaray ; José Elias Gomes ; Emanuel Carrilho ; Siu Mui Tsai
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 29, n. 1, p. -, 2006.
Resumo

Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm, ragweed, periwinkle and grape also infected by X. fastidiosa were also shown by the chromeazurol bioassay to produce siderophores. In order to correlate siderophore production with the presence of siderophore-related genes, a polymerase chain reaction (PCR) was developed using specific primers for the catechol-type ferric enterobactin receptor (pfeA) and the hydroxamate-type ferrisiderophore receptor (fiuA) genes of strain 9a5c. The PCR results confirmed our hypothesis by demonstrating that amplification products were detected in all strains except for those isolates that did not produce siderophores. (AU)

Processo FAPESP: 99/07748-1 - Identificação de genes envolvidos na biossíntese de metabólitos secundários peptídicos relacionados com a virulência em Xylella fastidiosa
Beneficiário:Flávia Teresa Hansen Pacheco
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 00/08045-3 - Investigação e caracterização in vitro de toxinas/oligopeptídeos não-ribossomais do sobrenadante de Xylella fastidiosa
Beneficiário:Maria Estela Stenico
Linha de fomento: Bolsas no Brasil - Pós-Doutorado