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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Fibroblast growth factor 18 regulates steroidogenesis in fetal bovine ovarian tissue in vitro

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Autor(es):
da Silva, Rubia Bueno [1] ; Yang, Ming Y. [2] ; Caixeta, E. S. [1] ; Castilho, Anthony C. [1] ; Amorim, R. L. [3] ; Price, C. A. [4] ; Fortune, J. E. [5, 2] ; Buratini, J. [5, 1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Inst Biociencias, Dept Fisiol, BR-18618000 Botucatu, SP - Brazil
[2] Cornell Univ, Dept Biomed Sci, Ithaca, NY - USA
[3] Univ Estadual Paulista, Fac Med Vet, Dept Clin Vet, Botucatu, SP - Brazil
[4] Univ Montreal, Fac Med Vet, Ctr Rech Reprod & Fertil, St Hyacinthe, PQ - Canada
[5] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 - USA
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Molecular Reproduction and Development; v. 86, n. 2, p. 166-174, FEB 2019.
Citações Web of Science: 0
Resumo

In cattle and other species, the fetal ovary is steroidogenically active before follicular development commences, and there is evidence that estradiol and progesterone inhibit follicle formation and activation. Estradiol levels decline sharply around the time of follicle formation. In the present study, we hypothesized that FGF10 and FGF18, which inhibit estradiol secretion from granulosa cells of antral follicles, also regulate fetal ovarian steroid production. Fetuses were collected at local abattoirs, and age determined by crown-rump length measurements. Real-time polymerase chain reaction assays with RNA extracted from whole ovaries revealed that the abundance of CYP19A1 messenger RNA (mRNA) decreased from 60 to 90 days of gestation, which is consistent with the decline in estradiol secretion previously observed. Immunohistochemistry revealed the presence of FGF18 in ovigerous cords in early gestation and in oocytes later in fetal age (>= 150 days). The abundance of FGF18 mRNA increased after Day 90 gestation. Addition of recombinant FGF18 to fetal ovarian pieces inhibited estradiol and progesterone secretion in vitro, whereas FGF10 was without effect. Consistent with these results, FGF18 decreased levels of mRNA for CYP19A1 and CYP11A1 in ovarian pieces in vitro. These data suggest that FGF18 may be an intraovarian factor that regulates steroidogenesis in fetal ovaries. (AU)

Processo FAPESP: 09/52300-2 - Participação do fator de crescimento fibroblástico 10 (FGF-10) no controle da foliculogênese pré-antral em fetos bovinos
Beneficiário:José Buratini Junior
Linha de fomento: Auxílio à Pesquisa - Regular