Programmed Death 1 (PD-1) and PD-1 Ligand (PD-L1) Expression in Chronic Apical Periodontitis

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Rezende Delgado, Ronan Jacques ^[1] ; Ramos Pinheiro, Claudia ^[1] ; Gasparoto, Thais Helena ^[1] ; Sipert, Carla Renata ^[2] ; de Moraes, Ivaldo Gomes ^[3] ; Garcia, Roberto Brandao ; Bramante, Clovis Monteiro ; Bernardineli, Norberti ; Nishiyama, Celso Kenji ^[4] ; da Silva, Joao Santana ^[5] ; Torres, Sergio Aparecido ^[1] ; Garlet, Gustavo Ponnpernnaier ^[1] ; Campanelli, Ana Paula ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru - Brazil

^[2] Univ Sao Paulo, Dent Sch, Dept Restorat Dent, Discipline Endodont, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Operat Dent, Endodont & Dent Mat, Bauru Sch Dent, Bauru - Brazil

^[4] CPO Uninga, Dent Postgrad Ctr, Dept Endodont Div, Bauru, SP - Brazil

^[5] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Biochem & Immunol, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN ENDODONTIC JOURNAL; v. 4, n. 1, p. 3-8, 2019.
• Citações Web of Science: 0

Resumo

Objective: This study aimed to examine programmed death protein 1 (PD-1) and programmed death ligand 1 (PD-L1) expression on leukocytes from chronic apical periodontitis, and to determine the levels of cytokines in the apical periodontitis lesions. Methods: Leukocytes from healthy gingival tissue (n=16) and chronic apical periodontitis (n=10) were evaluated using flow cytometry. The PD-1 and PDL-1 expressions were evaluated using flow cytometry. The cytokine levels were evaluated by enzyme-linked immunosorbent assay. Data were analyzed using one-way ANOVA. The statistical significance level was set at P<0.05. Results: Results showed that the apical periodontitis lesions are more infiltrated by PD-1(+) and PDL1(+) lymphocytes than the control samples. In addition, the PDL-1 expression was detected on macrophages in the apical periodontitis lesions, and was significantly higher compared to leukocytes from healthy gingival tissue. The IFN-gamma,TGF-beta, IL-10, and TNF-alpha levels were significantly higher in the apical periodontitis lesions compared to control samples. Conclusion:The PD-1, PD-L1, and CTLA-4 molecules are evident in apical periodontitis,and can be an important immune checkpoint in chronic periapical periodontitis. (AU)

• Processo FAPESP: 08/09973-3 - Envolvimento de sinais co-estimulatórios na progressão da periodontite apical crônica.
• Beneficiário: Ana Paula Campanelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular