Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232

Autor(es)

Espinal, P. ^{[1, 2]} ; Nucleo, E. ^[3] ; Caltagirone, M. ^[3] ; Marchetti, V. Mattioni ^[3] ; Fernandes, M. R. ^{[4, 1]} ; Biscaro, V ^[5] ; Rigoli, R. ^[5] ; Carattoli, A. ^[1] ; Migliavacca, R. ^[3] ; Villa, L. ^[1]

Afiliação do(s) autor(es)

^[1] Ist Super Sanita, Dept Infect Dis, Viale Regina Margherita 299, I-00161 Rome - Italy

^[2] Inst Invest Biomed St Pau IIB St Pau, Servei Microbiol Hosp Santa Creu & St Pau, Barcelona - Spain

^[3] Univ Pavia, Unit Microbiol & Clin Microbiol, Clin Surg Diagnost & Paediat Sci Dept, Pavia - Italy

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Sao Paulo - Brazil

^[5] Treviso Hosp, Microbiol Dept, Treviso - Italy

• Tipo de documento: Artigo Científico
• Fonte: Clinical Microbiology and Infection; v. 25, n. 3 MAR 2019.
• Citações Web of Science: 3

Resumo

Objectives: Genomic characterization of the internationally spread sequence type (ST) 16 carbapenemresistant Klebsiella pneumoniae. Methods: The complete genomes of three carbapenem producing ST16 K. pneumoniae from Italian patients were analysed by single-nucleotide polymorphism-based phylogeny, core genome multilocus sequence typing, resistance, plasmid, and virulence content and compared with ten genomes of ST16 strains isolated in other countries. Plasmids carrying blaNDM-1 or blaOXA-232 carbapenemase genes were assembled and sequences were analysed. Results: The internationally spread ST16 K. pneumoniae clone showed variability in terms of distribution of NDM-1 and OXA-232 type carbapenemases. In some ST16 strains, up to six plasmids can be simultaneously present in the same cell, including ColE-like plasmids carrying blaOXA-232 and IncF plasmids carrying blaNDM-1. The differences observed in plasmid, resistance, and virulence content and core genome suggested that there is not a unique, highly conserved ST16 clone, but instead different variants of this lineage circulate worldwide. Conclusions: The ST16 K. pneumoniae clone has spread worldwide and may become a high-risk clone. (c) 2018 Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases. (AU)

• Processo FAPESP: 17/16754-5 - Caracterização de Plasmídeos associado com a disseminação de resistência a colistina mcr-1 gene em Enterobacteriaceae clinicamente significantes de países da América do Sul e da Europa.
• Beneficiário: Miriam Rodriguez Fernandes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado