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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity

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Autor(es):
Honji, Renato Massaaki [1, 2] ; Caneppele, Danilo [3] ; Pandolfi, Matias [4, 5] ; Lo Nostro, Fabiana Laura [4, 5] ; Moreira, Renata Guimardes [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Rua Matao, Travessa 14, 321, BR-05508090 Sao Paulo - Brazil
[2] Univ Estadual Pordista UNESP, Ctr Aquicultura, Campus Jaboticabal, BR-14884900 Sao Paulo - Brazil
[3] CESP, Unidade Hidrobiol & Aquicultura, Rodovia Tamoios, Km 38, BR-12260000 Sao Paulo - Brazil
[4] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Ciudad Univ C1428EHA, Buenos Aires, DF - Argentina
[5] UBA, CONICET, IBBEA, Ciudad Univ C1428EHA, Buenos Aires, DF - Argentina
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: General and Comparative Endocrinology; v. 273, n. SI, p. 73-85, MAR 1 2019.
Citações Web of Science: 1
Resumo

This study evaluated by immunohistochemical and Western blot methods, the distribution of two distinct gonadotropin-releasing hormones (GnRHs), corresponding to catfish GnRH (cfGnRH or GnRH1) and chicken-II GnRH (cGnRH-II or GnRH2), in Steindachneridion parahybae females in captivity, focusing these analyses on the reproductive cycle by semi-quantification of optical density (OD). Further, we found that the GnRH neuronal systems co-localized with their respective GnRH-associated peptides (GAPs). A group of neurons immunoreactive (ir) to GnRH1 were identified along the ventral region of the olfactory bulb (vOB) in the telencephalon (vTel) and in the main areas of the diencephalon (especially the medial basal hypothalamus, HBM), including fibers extending into the pituitary gland. In contrast, GnRH2 neurons were confined to the midbrain tegmentum, close to the ventricular surface, without projections to the pituitary gland. Moreover, a cfGAP (GnRH1)-specific band (9 kDa) was identified in the brain and pituitary gland, while a cGAP-II (GnRH2)-specific band (26 kDa) was observed only in the brain extract. During the reproductive cycle, GnRH1-ir presented greater OD values at the vitellogenic and regression stages than at the previtellogenic stage and after artificially induced to spawn. Larger GnRH2-ir neurons were observed during the reproductive cycle, but a higher OD was identified only in the regression stage compared with the other maturation stages. Finally, GnRH1 axons were found to be directed towards the pituitary, and this GnRH type, which is probably the hypophysiotropic form, can contribute to the reproductive dysfunction that occurs in S. parahybae females in captivity, whereas GnRH2 may act as a neuromodulator and/or neurotransmitter. (AU)

Processo FAPESP: 08/57687-0 - Effects of global climate change of the Brazilian fauna: a conservation physiology approach
Beneficiário:Carlos Arturo Navas Iannini
Linha de fomento: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
Processo FAPESP: 07/55494-7 - Controle do eixo hipotálamo-hipófise-gônadas do surubim do Paraíba Steindachneridion Parahybae (Siluriformes: Pimelodidae) em relação ao ciclo reprodutivo e à reprodução induzida em cativeiro
Beneficiário:Renato Massaaki Honji
Linha de fomento: Bolsas no Brasil - Doutorado