EP80317 Restrains Inflammation and Mortality Caused by Scorpion Envenomation in Mice

Autor(es)

Zoccal, Karina F. ^{[1, 2]} ; Gardinassi, Luiz G. ^[1] ; Bordon, Karla C. F. ^[3] ; Arantes, Eliane C. ^[3] ; Marleau, Sylvie ^[4] ; Ong, Huy ^[4] ; Faccioli, Lucia H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Ctin Toxicol & Bromatol, Ribeirao Preto - Brazil

^[2] Ctr Univ Barao Maua, Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Ribeirao Preto - Brazil

^[4] Univ Montreal, Fac Pharm, Montreal, PQ - Canada

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PHARMACOLOGY; v. 10, MAR 1 2019.
• Citações Web of Science: 1

Resumo

Over 1 million cases of scorpion stings are estimated every year, whereas current treatment is limited to antivenom serum combined with supportive therapy. Tityus serrulatus scorpion venom (TsV) is composed of diverse molecules, including toxins that induce a catecholamine storm and mediate classical symptoms of scorpion envenomation. However, the same toxins promote an intense inflammatory response coordinated by innate immune cells, such as macrophages, contributing significantly to the lung edema and mortality caused by TsV injection. Macrophages sense TsV via innate immune receptors, including TLR2, TLR4, and CD14 that promote inflammation and mortality via PGE(2)/cAMP/PKA/NF-kappa B/ IL-1 beta axis. The scavenger receptor CD36 also recognizes TsV, but in contrast to the other receptors, it drives the production of leukotriene B-4 (LTB4). This lipid mediator operates via BLT1 receptor to reduce cAMP production and consequently IL-1 beta release, which results in resistance to fatal outcomes of experimental scorpion envenomation. EP80317 is an hexapeptide that serves as a ligand for CD36 and features protective effects under conditions such as atherosclerosis and vascular inflammation. In this study, we evaluated the effects of EP80317 treatment during experimental scorpion envenomation. EP80317 treatment suppressed mouse peritoneal macrophage production of IL-1 beta, IL-6, tumor necrosis factor (TNF-alpha), CCL3, and PGE(2) in vitro. EP80317 treatment also boosted the production of LTB4 and IL-10 in response to TsV. Importantly, EP80317 restrained lung inflammation and mortality caused by TsV in vivo. Taken together, these data indicate a strong therapeutic potential of EP80317 as a supportive treatment to control inflammation induced by scorpion envenomation. (AU)

• Processo FAPESP: 14/07125-6 - Novos aspectos funcionais dos eicosanóides
• Beneficiário: Lúcia Helena Faccioli
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/03332-7 - Estudo da relação entre ativação do inflamassoma e produção de mediadores lipídicos com a inflamação pulmonar induzida por veneno de escorpião com e sem hialuronidase
• Beneficiário: Karina Furlani Zoccal
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/00658-1 - Novos aspectos funcionais dos eicosanóides
• Beneficiário: Lúcia Helena Faccioli
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários