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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The evolution, distribution and diversity of endogenous circoviral elements in vertebrate genomes

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Autor(es):
Dennis, Tristan P. W. [1] ; de Souza, William Marciel [2, 1] ; Marsile-Medun, Soledad [1, 3] ; Singer, Joshua B. [1] ; Wilson, Sam J. [1] ; Gifford, Robert J. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Glasgow, MRC, Ctr Virus Res, 464 Bearsden Rd, Glasgow, Lanark - Scotland
[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Virol Res Ctr, Ribeirao Preto - Brazil
[3] Agrocampus Ouest, 65 Rue St Brieuc, F-35000 Rennes - France
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: VIRUS RESEARCH; v. 262, n. SI, p. 15-23, MAR 2019.
Citações Web of Science: 2
Resumo

Circoviruses (family Circoviridae) are small, non-enveloped viruses that have short, single-stranded DNA genomes. Circovirus sequences are frequently recovered in metagenomic investigations, indicating that these viruses are widespread, yet they remain relatively poorly understood. Endogenous circoviral elements (CVe) are DNA sequences derived from circoviruses that occur in vertebrate genomes. CVe are a useful source of information about the biology and evolution of circoviruses. In this study, we screened 362 vertebrate genome assemblies in silica to generate a catalog of CVe loci. We identified a total of 179 CVe sequences, most of which have not been reported previously. We show that these CVe loci reflect at least 19 distinct germline integration events. We determine the structure of CVe loci, identifying some that show evidence of potential functionalization. We also identify orthologous copies of CVe in snakes, fish, birds, and mammals, allowing us to add new calibrations to the timeline of circovirus evolution. Finally, we observed that some ancient CVe group robustly with contemporary circoviruses in phylogenies, with all sequences within these groups being derived from the same host class or order, implying a hitherto underappreciated stability in circovirus-host relationships. The openly available dataset constructed in this investigation provides new insights into circovirus evolution, and can be used to facilitate further studies of circoviruses and CVe. (AU)

Processo FAPESP: 17/13981-0 - Caracterização, genômica e diagnóstico de vírus com importância em saúde pública no Brasil por sequenciamento de alto desempenho
Beneficiário:William Marciel de Souza
Linha de fomento: Bolsas no Brasil - Pós-Doutorado