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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Combination of Natural Extracts and Photobiomodulation in Keratinocytes Subjected to UVA Radiation

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Autor(es):
Silva, Elineides Santos [1] ; Machado, Gabriela Benedito [1] ; Lino-dos-Santos-Franco, Adriana [1] ; Pavani, Christiane [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Nove de Julho UNINOVE, Post Grad Program Biophoton Appl Hlth Sci, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Photochemistry and Photobiology; v. 95, n. 2, p. 644-649, MAR-APR 2019.
Citações Web of Science: 0
Resumo

Natural extracts (NE) with antioxidant properties can minimize the effects of photoaging. Photobiomodulation (PBM) has proven to be a useful tool for the modulation of cell metabolism. Here, we investigate the associations of antioxidants with PBM with the aim of promoting skin rejuvenation. We began with standardization of the experimental protocol. Extracts of chamomile, rosemary, blueberry, green tea, figs, pomegranate and nutwood were tested. A custom irradiation system (366 +/- 10 nm) was used to simulate sun exposure. A light emitting diode system (640 +/- 12.5 nm) was used for PBM. Viability assessments were performed by the (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) MTT assay method. Based on the results, radiant exposure to UVA was defined as 9 and 1 J cm(-2) for PBM. Extract concentrations were established on the basis of dark toxicities, which ranged from 0.01% to 0.3%. The data show that PBM is a promising therapy to restore keratinocytes after UVA damage; however, the detailed mechanism and effects require further exploration. Moreover, although the combination of PBM with NE may be a useful strategy, the choice of a NE is challenging, since the working concentration and other properties, such as photosensitivity, may bring about unwanted results. (AU)

Processo FAPESP: 12/50680-5 - Fotossensibilização nas ciências da vida
Beneficiário:Mauricio da Silva Baptista
Linha de fomento: Auxílio à Pesquisa - Temático