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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Polysaccharides from chayote enhance lipid efflux and regulate NLRP3 inflammasome priming in macrophage-like THP-1 cells exposed to cholesterol crystals

Texto completo
Autor(es):
Castro-Alves, Victor Costa [1, 2, 3, 4] ; Shiga, Tania Misuzu [1, 2, 3, 4] ; Oliveira do Nascimento, Joao Roberto [1, 5, 2, 3, 4]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food Sci & Expt Nutr, Sao Paulo, SP - Brazil
[2] Sao Paulo Res Fdn, Food Res Ctr FoRC, CEPID, FAPESP, Res Ctr, Sao Paulo - Brazil
[3] Sao Paulo Res Fdn, Food Res Ctr FoRC, CEPID, FAPESP, Innovat Ctr, Sao Paulo - Brazil
[4] Sao Paulo Res Fdn, Food Res Ctr FoRC, CEPID, FAPESP, Disseminat Ctr, Sao Paulo - Brazil
[5] Univ Sao Paulo, Food & Nutr Res Ctr NAPAN, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Macromolecules; v. 127, p. 502-510, APR 15 2019.
Citações Web of Science: 1
Resumo

The contribution of dietary fiber to decrease the risk of atherosclerosis may occur through other mechanisms besides the increased excretion of cholesterol. Although macrophages are crucial for lipid clearance, the excessive uptake of cholesterol crystals (CC) by these cells induce NLRP3 inflammasome and foam cell formation. Thus, we investigated whether the water-soluble DF from chayote (WSP) regulate CC-pretreated macrophage-like THP-1 cells. Linkage analysis indicated that WSP is composed mainly of pectic homogalacturonan and highly branched type I rhamnogalacturonan as well as hemicellulosic material including glucomannan, xyloglucan, and glucurono(arabino)xylan. WSP reduced interleukin (IL)-1 beta and chemokine release in CC-pretreated macrophages. Notably, WSP also reduced lipid accumulation in cells previously exposed to CC. Furthermore, WSP up regulated liver X receptor alpha expression, which may account for increased lipid efflux, and reduced matrix metallopeptidase 9 expression. WSP also reduced active caspase-1 protein levels, and downregulated NLRP3 and IL-1 beta gene expression in CC-pretreated cells, suggesting that this polysaccharide fraction regulates the priming signals required for NLRP3 inflammasome activation. Thus, WSP regulate lipid efflux and suppress inflammasome priming in macrophages, suggesting that the health benefits of this dietary fiber could go beyond its physical properties on the gastrointestinal tract. (C) 2019 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 16/05083-0 - Efeito de polissacarídeos de origem fúngica e vegetal do tipo não-amido sobre a função de macrófagos
Beneficiário:Victor Costa Castro Alves
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
Beneficiário:Bernadette Dora Gombossy de Melo Franco
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs