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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Oxidative stress induced by self-adhesive resin cements affects gene expression, cellular proliferation and mineralization potential of the MDPC-23 odontoblast-like cells

Texto completo
Autor(es):
Palacio Alvarez, Marcela Maciel [1] ; de Carvalho, Rafael Guzella [1] ; de Arruda Barbosa, Silvana Coelho [2] ; Polassi, Mackeler Ramos [2] ; Nascimento, Fabio Dupart [3] ; Perlatti D'Alpino, Paulo Henrique [2] ; dos Santos Tersariol, Ivarne Luis [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil
[2] Univ Anhanguera Sao Paulo UNIAN SP, Biotechnol & Innovat Hlth Program, Sao Paulo, SP - Brazil
[3] Univ Mogi das Cruzes, Interdisciplinary Ctr Biochem Invest, Mogi Das Cruzes, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Dental Materials; v. 35, n. 4, p. 606-616, APR 2019.
Citações Web of Science: 0
Resumo

Objective. Clinical issues have been raised about problems related to cytotoxic effects caused when applying self-adhesive cement. It was hypothesized that byproducts eluted from self-adhesive cements modulate oxidative stress response, the gene expression of signaling pathways of inflammatory process/transcriptional activators, and the expression and activity of interstitial collagenases, and modify the phenotypic characteristics of cellular proliferation and mineral deposition in odontoblastic-like cells. Methods. Cements (MaxCem Elite {[}MAX] and RelyX U200 {[}U200)]) were mixed, dispensed into moulds, and photoactivated according to the manufacturers' instructions. Immortalized rat odontoblast-like cells (MDPC-23) were cultured and exposed to polymerized specimens of cements for 4 h. Reactive oxidative specimen production and quantification of gene expression were evaluated. Cell proliferation assay and alizarin red staining were also performed to evaluate the disturbance induced by the cements on cellular proliferation and mineralization. Results. Despite their cytotoxic effects, both self-adhesive cements influenced the metabolism in the odontoblast cells on different scales. MAX induced significantly higher oxidative stress in odontoblast cells than U200. Gene expression varied as a function of exposure to self-adhesive cements; MAX induced the expression of pro-inflammatory cytokines such as TNF-alpha, whereas U200 downregulated, virtually depleted TNF-alpha expression, also inducing overexpression of the transcriptional factor Runx2. Overexpression of hemeoxygenase-1 (HO-1) and thioredoxin reductase 1 (TRXR1) occurred after exposure to both cements, antioxidant genes that are downstream of Keap1-Nrf2-ARE system. MAX significantly induced the overexpression of collagenase MMP-1, and U200 induced the expression of gelatinase MMP-2. MAX significantly inhibited cell proliferation whereas U200 significantly activated cell proliferation. Alizarin red staining revealed significantly decreased mineral deposition especially when exposed to MAX. Significance. These results support the hypothesis that byproducts of different self-adhesive cements play important roles in the highly orchestrated process which ultimately affect the cellular proliferation and the mineral deposition in odontoblastic-like cells, possibly delaying the reparative dentin formation after cementation of indirect restorations, especially on recently exposed dentin preparations. (C) 2019 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 13/05822-9 - Receptores ativados por proteases (PARs) no complexo dentina-polpa: identificação, modulação e sinalização celular durante o desenvolvimento da doença cárie
Beneficiário:Fábio Dupart Nascimento
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 15/03964-6 - Glicosaminoglicanos e proteoglicanos: relação estrutura e função
Beneficiário:Helena Bonciani Nader
Linha de fomento: Auxílio à Pesquisa - Temático