Systems Biology Reveals NR2F6 and TGFB1 as Key Regulators of Feed Efficiency in Beef Cattle

Autor(es)

Alexandre, Pamela A. ^{[1, 2]} ; Naval-Sanchez, Marina ^[2] ; Porto-Neto, Laercio R. ^[2] ; Ferraz, Jose Bento S. ^[1] ; Reverter, Antonio ^[2] ; Fukumasu, Heidge ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Vet Med, Coll Anim Sci & Food Engn, Pirassununga - Brazil

^[2] CSIRO, Agr & Food, Brisbane, Qld - Australia

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN GENETICS; v. 10, MAR 22 2019.
• Citações Web of Science: 1

Resumo

Systems biology approaches are used as strategy to uncover tissue-specific perturbations and regulatory genes related to complex phenotypes. We applied this approach to study feed efficiency (FE) in beef cattle, an important trait both economically and environmentally. Poly-A selected RNA of five tissues (adrenal gland, hypothalamus, liver, skeletal muscle and pituitary) of eighteen young bulls, selected for high and low FE, were sequenced (Illumina HiSeq 2500, 100 bp, pared-end). From the 17,354 expressed genes considering all tissues, 1,335 were prioritized by five selection categories (differentially expressed, harboring SNPs associated with FE, tissue-specific, secreted in plasma and key regulators) and used for network construction. NR2F6 and TGFB1 were identified and validated by motif discovery as key regulators of hepatic inflammatory response and muscle tissue development, respectively, two biological processes demonstrated to be associated with FE. Moreover, we indicated potential biomarkers of FE, which are related to hormonal control of metabolism and sexual maturity. By using robust methodologies and validation strategies, we confirmed the main biological processes related to FE in Bos indicus and indicated candidate genes as regulators or biomarkers of superior animals. (AU)

• Processo FAPESP: 17/14707-0 - Análises ômicas multi-teciduais para caracterizar a eficiência alimentar em bovinos de corte
• Beneficiário: Pâmela Almeida Alexandre
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/22276-3 - Abordagem de Biologia de Sistemas Para a Determinação de Mecanismos Moleculares Associados à Eficiência Alimentar de Bovinos Nelore
• Beneficiário: Pâmela Almeida Alexandre
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/07566-2 - Genômica aplicada à produção de ruminantes
• Beneficiário: José Bento Sterman Ferraz
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/02493-7 - Neoplasias mamárias de cadelas e a teoria das células-tronco cancerosas: uma abordagem comparada e translacional
• Beneficiário: Heidge Fukumasu
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores