Influence of Pluronic F127 microenvironments on the photochemical nitric oxide release from S-nitrosoglutathione

Autor(es)

Picheth, Guilherme F. ^[1] ; Marini, Thatiane C. ^[1] ; Taladriz-Blanco, Patricia ^{[1, 2]} ; Shimamoto, Gustavo G. ^[1] ; dos Santos, Giovanna J. V. P. ^[1] ; Meneau, Florian ^[3] ; de Oliveira, Marcelo G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, UNICAMP, CP 6154, BR-13083970 Campinas, SP - Brazil

^[2] Adolphe Merkle Inst, Chemin Verdiers 4, CH-1700 Fribourg - Switzerland

^[3] Brasilian Synchroton Light Lab, LNLS, CP 6192, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Colloid and Interface Science; v. 544, p. 217-229, MAY 15 2019.
• Citações Web of Science: 1

Resumo

Poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) (F127) hydrogels have been used to deliver nitric oxide (NO) topically in biomedical applications. Here, the effect of F127 microenvironments on the photochemical NO release from S-nitrosoglutathione (GSNO) was investigated in F127 solutions 7.6 wt% 15 wt% and 22.5 wt% at 15 degrees C and 37 degrees C. Small-angle X-ray Scattering (SAXS) and Differential Scanning Calorimetry (DSC) measurements, along with proton Nuclear Magnetic Resonance (H-1 NMR) spectral shifts and T-2 relaxation data at six different concentration-temperature conditions, allowed identifying F127 microphases characterized by: a sol phase of unimers; micelles in non-defined periodic order, and a gel phase of cubic packed micelles. Kinetic measurements showed that GSNO photodecompositon proceeds faster in micellized F127 where GSNO is segregated to the intermicellar microenvironment. Real time kinetic monitoring of NO release and T-2 relaxation profiles showed that NO is preferentially partitioned into the hydrophobic PPO cores of the F127 micelles, with the consequent decrease in its rate of release to the gas phase. These results show that F127 microphases affect both the kinetics of GSNO photodecomposition and the rate of NO escape and can be used to modulate the photochemical NO delivery from F127/GSNO solutions. (C) 2019 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 16/02414-5 - Biomateriais absorvíveis e tópicos para a liberação localizada de óxido nítrico
• Beneficiário: Marcelo Ganzarolli de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/19253-7 - Hidrogéis termosensíveis de Pluronic F127 funcionalizados com grupos terminais liberadores de óxido nítrico
• Beneficiário: Guilherme Fadel Picheth
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/03266-4 - Nanocarregadores termosensíveis de PVA-F-127 para a liberação fotoquímica de óxido nítrico
• Beneficiário: Thatiane Cristine Camargo Marini
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/04615-0 - Membranas de colágeno doadoras de óxido nítrico para regeneração óssea
• Beneficiário: Giovanna Jardim Vieira Pereira dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/07173-8 - Nanopartículas e hidrogéis para a liberação fotoquímica de óxido nítrico
• Beneficiário: Patricia Taladriz Blanco
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado