Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

AKT/protein kinase B associates with beta-actin in the nucleus of melanoma cells

Texto completo
Autor(es):
Coa, Larissa Leggieri [1] ; Abreu, Thiago Ferreira [2] ; Tashima, Alexandre Keiji [2] ; Green, Janaina [1] ; Pascon, Renata Castiglioni [1] ; Vallim, Marcelo Afonso [1] ; Machado-, Jr., Joel [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo UNIFESP, Dept Biol Sci, Diadema, SP - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Biochem, Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: BIOSCIENCE REPORTS; v. 39, n. 1 JAN 31 2019.
Citações Web of Science: 1
Resumo

The serine-threonine kinase AKT/PKB is a critical regulator of various essential cellular processes, and dysregulation of AKT has been implicated in many diseases, including cancer. Despite AKT action is known to function mainly in the cytoplasm, AKT has been reported to translocate to the nucleus. However, very little is known about the mechanism required for the nuclear import of AKT as well as its function in this cellular compartment. In the present study, we characterized the presence of endogenous nuclear AKT in human melanoma cells and addressed the possible role of AKT by exploring its potential association with key interaction nuclear partners. Confocal and Western blot analyses showed that both phosphorylated and non-phosphorylated forms of AKT are present in melanoma cells nuclei. Using mass spectrometry in combination with protein-crosslinking and co-immunoprecipitation, we identified a series of putative protein partners of nuclear AKT, including heterogeneous nuclear ribonucleoprotein (hnRNP), cytoskeleton proteins beta-actin, gamma-actin, beta-actin-like 2 and vimentin. Confocal microscopy and biochemical analyses validated beta-actin as a new nuclear AKT-interacting partner. Cofilin and active RNA Polymerase II, two proteins that have been described to interact and work in concert with nuclear actin in transcription regulation, were also found associated with nuclear AKT. Overall, the present study uncovered a yet unrecognized nuclear coupling of AKT and provides insights into the involvement of AKT in the interaction network of nuclear actin. (AU)

Processo FAPESP: 12/19321-9 - Caracterização proteômica e peptidômica dos venenos de aranhas brasileiras por espectrometria de massas
Beneficiário:Alexandre Keiji Tashima
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/50953-9 - Relevância funcional da quinase Akt/PKB nuclear na regulação da resistência ao anoikis em linhagens celulares de melanoma
Beneficiário:Joel Machado Junior
Linha de fomento: Auxílio à Pesquisa - Regular