Lipid-mediated growth of SrCO3/CaCO3 hybrid films as bioactive coatings for Ti surfaces

Autor(es)

Cruz, M. A. E. ^[1] ; Zanatta, M. B. T. ^[1] ; da Veiga, M. A. M. S. ^[1] ; Ciancaglini, P. ^[1] ; Ramos, A. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 99, p. 762-769, JUN 2019.
• Citações Web of Science: 1

Resumo

SrCO3 is frequently used as Sr2+ source in ceramic cements, but its application as bioactive coating for metallic implants has not been explored yet. Aiming at rapid osteointegration and because of the well-known Sr2+ effects on bone metabolism, researchers have sought to design Sr2+-containing biomaterials. In this context, developing simple techniques to prepare Sr2+-based coatings is a must nowadays. Here, we describe the use of a bioinspired lipid-mediated approach to grow SrCO3 hybrid films on Ti surfaces at room temperature. To obtain these coatings, we applied the Langmuir-Blodgett technique to deposit phospholipid films with high degree of organization on Ti. In this way, we expected that controlled SrCO3 crystal growth could be templated by the array of nucleation points arising from electrostatic interaction between Sr2+ and the phospholipid polar heads. To control surface composition and the amount of Sr2+ released from the coatings, we also promoted CaCO3 co-precipitation in the hybrid films. We characterized the hybrid coatings in terms of morphology, chemical structure, wettability, and ability to release Sr2+ upon immersion in biological medium. In vitro osteoblast culture on mixed SrCO3/CaCO3 films revealed that the osteogenic response depended on surface composition, as indicated by alkaline phosphatase activity overexpression, which is an early indicator of osteoblast differentiation. Results showed that the mixed SrCO3/CaCO3 hybrid film created a synergic environment for osteoblasts, and that proper Sr2+ release associated with a Ca2+-rich environment might have optimized the Sr2+-anabolic effect. In conclusion, we have proposed a bioinspired and versatile technique to grow hybrid films that can control surface composition and Sr2+ release. Our results open an opportunity to explore the use of SrCO(3)Z-based coatings for rapid metallic implant osteointegration. (AU)

• Processo FAPESP: 17/08892-9 - Superfícies bioativas obtidas a partir de filmes Langmuir-Blodgett e biominerais
• Beneficiário: Ana Paula Ramos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/21236-0 - Sistemas miméticos de vesículas da matriz extracellular (MVs) para o estudo da regulação do processo de biomineralização: proteolipossomos contendo NPP1 e Anexina V
• Beneficiário: Pietro Ciancaglini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/08774-0 - Modificação de superfícies de Ti com filmes híbridos Langmuir-Blodgett contendo biominerais e ranelato de estrôncio para liberação controlada de Sr2+
• Beneficiário: Marcos Antonio Eufrásio Cruz
• Modalidade de apoio: Bolsas no Brasil - Mestrado