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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cation-doped bioactive ceramics: In vitro bioactivity and effect against bacteria of the oral cavity

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Siqueira, Renato Luiz [1, 2] ; Silva Alves, Patricia Francieli [3] ; Moraes, Thais da Silva [3] ; Casemiro, Luciana Assirati [3] ; da Silva, Sidney Nicodemos [2] ; Peitl, Oscar [1] ; Gomes Martins, Carlos Henrique [3] ; Zanotto, Edgar Dutra [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Engn Mat, Lab Mat Vitreos, Rodovia Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil
[2] Ctr Fed Educ Tecnol Minas Gerais, Dept Engn Mat, Ave Amazonas 5253, BR-30421169 Belo Horizonte, MG - Brazil
[3] Univ Franca, Lab Pesquisa Microbiol Aplicada, Ave Doutor Armando de Sales Oliveira 201, BR-14404600 Franca, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: CERAMICS INTERNATIONAL; v. 45, n. 7, A, p. 9231-9244, MAY 2019.
Citações Web of Science: 2

We synthesized, using a solid-state reaction, bioactive ceramics of the SiO2-CaO-Na2O-P2O5 system doped with up to 3 mol% of Ag, Mg, Sr, Zn, and Ga, elements that were used as therapeutic agents. On evaluating the in vitro bioactivity of our crystalline samples using the ICG-TC04 method (so far only tested for glassy materials), we observed that those ceramics containing up to 0.5 mol% dopant exhibited hydroxycarbonate apatite (HCA) layer formation on their surfaces in a 24-h in vitro assay - the same time observed for Bioglass{*}, the material considered to be ``gold standard{''} in terms of bioactivity. The antibacterial activity of these ceramics was confirmed against 23 oral bacteria, related to caries and endodontic infections, by employing three different methods. For the agar dilution method, using a concentration range of 0.5-24 mg/mL, various bacteria were found to be susceptible to the materials - the lowest minimum inhibitory concentration (MIC) values were achieved for the doped samples, particularly those containing Ag. In the biofilm-forming capability assay, we did not observe any viable cells after 24 h incubation. Finally, the direct contact assay confirmed that all the samples showed a stronger antibacterial effect, promoting a significant reduction in the number of viable cells after only 10 min in contact with the microorganisms. This result indicates that the tested materials have an intrinsic antibacterial activity, and do not need to be doped, except for some specific bacteria or depending on how they will be used (extracts or in direct contact). Therefore, besides easy production and in vitro bioactivity, all tested materials demonstrated a considerable antibacterial effect against representative bacteria, stimulating future studies involving their application as topical endodontic disinfectants or in dental prophylaxis procedures. (AU)

Processo FAPESP: 13/07793-6 - CEPIV - Centro de Ensino, Pesquisa e Inovação em Vidros
Beneficiário:Edgar Dutra Zanotto
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 14/07483-0 - Avaliação do potencial antibacteriano do biosilicato® contendo metais com ação biológica
Beneficiário:Patrícia Francieli Silva Alves
Linha de fomento: Bolsas no Brasil - Iniciação Científica