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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A cheap and sensitive method for imaging Gracilaria (Rhodophyta, Gracilariales) growth

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Autor(es):
Alves-Lima, Cicero [1] ; Azevedo Teixeira, Andre Reis [1] ; Hotta, Carlos Takeshi [1] ; Colepicolo, Pio [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05599970 Sao Paulo, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF APPLIED PHYCOLOGY; v. 31, n. 2, p. 885-892, APR 2019.
Citações Web of Science: 0
Resumo

Growth rate is an informative measurement for the culture of any organism, as it provides cues about fitness and allows selection of strains. For seaweeds, the gain of mass has been the most used methodology at the laboratory, but it can be laborious and increase the contamination. In this work, we present an easy, accessible, and sensitive method that uses imaging to measure the growth rate at a laboratory scale of three Gracilaria species (Rhodophyta, Gracilariales): G. caudata J. Agardh, G. domingensis (Kutzing) Sonder ex Dickie, and G. tenuistipitata C.F.Chang \& B.M.Xia. A CMOS camera with a flex clamp and a macro lens was attached to a common retort stand in order to measure the algae thalli from the bottom of a Petri dish. Then, the images were batch processed for area and length measurement and correlated to mass. A high correlation between mass and area was verified for all three species, but not for length (G. domingensis, R-2 = 0.68). G. tenuistipitata had the highest growth rate in area (17.9% day(-1)) and G. domingensis the lowest (3.1% day(-1)). Correlation differences between area and mass can be due to species-specific morphology. Area measurement can efficiently replace weighing. The present method is highly adaptable to many experimental setups, uses a cheap camera and free software, is not labor intensive and does not require much computer knowledge. (AU)

Processo FAPESP: 13/05301-9 - Estudo sobre as interações entre o relógio biológico e a dessecação em eucariotos fotossintetizantes
Beneficiário:Cícero Alves Lima Júnior
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 15/06260-0 - Relógios biológicos específicos em órgãos e tecidos de gramíneas C4
Beneficiário:Carlos Takeshi Hotta
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
Processo FAPESP: 16/06931-4 - Biodiversidade e prospecção de algas de águas tropicais e da Antártica marítima
Beneficiário:Pio Colepicolo Neto
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático