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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Layers of Cryptic Genetic Variation Underlie a Yeast Complex Trait

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Autor(es):
Lee, Jonathan T. [1] ; Coradini, V, Alessandro L. ; Shen, Amy [2] ; Ehrenreich, Ian M. [2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Southern Calif, Dept Biol Sci, Mol & Computat Biol Sect, Los Angeles, CA 90089 - USA
[2] Coradini, Alessandro L., V, Univ Southern Calif, Dept Biol Sci, Mol & Computat Biol Sect, Los Angeles, CA 90089 - USA
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Genetics; v. 211, n. 4, p. 1469-1482, APR 2019.
Citações Web of Science: 2
Resumo

Cryptic genetic variation may be an important contributor to heritable traits, but its extent and regulation are not fully understood. Here, we investigate the cryptic genetic variation underlying a Saccharomyces cerevisiae colony phenotype that is typically suppressed in a cross of the laboratory strain BY4716 (BY) and a derivative of the clinical isolate 322134S (3S). To do this, we comprehensively dissect the trait's genetic basis in the BYx3S cross in the presence of three different genetic perturbations that enable its expression. This allows us to detect and compare the specific loci that interact with each perturbation to produce the trait. In total, we identify 21 loci, all but one of which interact with just a subset of the perturbations. Beyond impacting which loci contribute to the trait, the genetic perturbations also alter the extent of additivity, epistasis, and genotype-environment interaction among the detected loci. Additionally, we show that the single locus interacting with all three perturbations corresponds to the coding region of the cell surface gene . While nearly all of the other remaining loci influence transcription in cis or trans, the perturbations tend to interact with loci in different pathways and subpathways. Our work shows how layers of cryptic genetic variation can influence complex traits. Here, these layers mainly represent different regulatory inputs into the transcription of a single key gene. (AU)

Processo FAPESP: 16/21176-8 - Identificação de genes que modulam a relação genótipo-fenótipo em Saccharomyces cerevisiae.
Beneficiário:Alessandro Luis Venega Coradini
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado